EV71 VP1 monoclonal antibody, clone 3D7
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Specification
Product Description
Mouse monoclonal antibody raised against a full length recombinant virus EV71 VP1.
Immunogen
EV71 VP1 (AAP82198.1, 1 a.a. ~ 297 a.a) recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
GDRVADVIESSIGDSVSRALTQALPAPTGQNTQVSSHRLDTGEVPALQAAEIGASSNTSDESMIETRCVLNSHSTAETTLDSFFSRAGLVGEIDLPLEGTTNPNGYANWDIDITGYAQMRRKVELFTYMRFDAEFTFVACTPTGGVVPQLLQYMFVPPGAPKPDSRESLAWQTATNPSVFVKLTDPPAQVSVPFMSPASAYQWFYDGYPTFGEHKQEKDLEYGACPNNMMGTFSVRTVGSSKSKYPLVVRIYMRMKHVRAWIPRPMRNQNYLFKANPNYAGNSIKPTGTSRTAITTL
Host
Mouse
Reactivity
Viruses
Form
Liquid
Isotype
IgG1, kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against EV71 VP1 (59.07 KDa) .
Recommend Usage
The optimal working dilution should be determined by the end user.
Storage Buffer
In PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Recombinant protein)
ELISA
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Publication Reference
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Construction and characterization of an infectious cDNA clone of enterovirus 71: a rapid method for rescuing infectious virus based on stable cells expressing T7 polymerase.
Meixian Fu, Jinjin Bai, Shuang Gao, Zhangmei Chang, Xin Zhou, Jian-Er Long.
Archives of Virology 2021 Feb; 166(2):627.
Application:IF, Human, SH-SY5Y cells.
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Enterovirus A71 VP1 Variation A289T Decreases the Central Nervous System Infectivity via Attenuation of Interactions between VP1 and Vimentin In Vitro and In Vivo.
Zhu H, Cao Y, Su W, Huang S, Lu W, Zhou Y, Gao J, Zhao W, Zhang B, Wu X.
Viruses 2019 May; 11(5):E467.
Application:WB, Human, Human brain microvascular endothelial cells.
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Characteristics of Enterovirus 71-induced cell death and genome scanning to identify viral genes involved in virus-induced cell apoptosis.
Bai J, Chen X, Liu Q, Zhou X, Long JE.
Virus Research 2019 Mar; 265:104.
Application:WB, Human, HeLa, SH-SY5Y, RD cells.
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Cloning and Expression of Enterovirus 71 Capsid Protein 1 in a Probiotic Bifidobacterium pseudocatenulatum.
Thinbanmai T, Lulitanond V, Mayo B, Lulitanond A, Panya M.
Letters in Applied Microbiology 2019 Jan; 68(1):9.
Application:WB, Recombinant protein.
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Enterovirus 71 protease 2Apro and 3Cpro differentially inhibit the cellular endoplasmic reticulum-associated degradation (ERAD) pathway via distinct mechanisms, and enterovirus 71 hijacks ERAD component p97 to promote its replication.
Wang T, Wang B, Huang H, Zhang C, Zhu Y, Pei B, Cheng C, Sun L, Wang J, Jin Q, Zhao Z.
PLoS Pathog 2017 Oct; 13(10):e1006674.
Application:IF, WB, Human, Rhabdomyosarcoma (RD) cells.
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Validation-based insertional mutagenesisfor identification of Nup214 as a host factor for EV71 replication in RD cells.
Wang B, Zhang X, Zhao Z.
Biochemical and Biophysical Research Communications 2013 Aug; 437(3):452.
Application:WB, Human, RD cells.
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Construction and characterization of an infectious cDNA clone of enterovirus 71: a rapid method for rescuing infectious virus based on stable cells expressing T7 polymerase.
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