SRC monoclonal antibody, clone 17AT28
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More Files
- More Functions
Western Blot (Cell lysate)
The SRC monoclonal antibody, clone 17AT28 (Cat # MAB1155) is used in Western blot to detect SRC in Jurkat cell lysate.
Western Blot (Transfected lysate)
FG Pancreatic Carcinoma Cell Lines stably expressing vector along (FG-V) the beta3 integrin subunit (FG-beta3) or a beta3 truncation mutant (FG-759x) . SRC monoclonal antibody, clone 17AT28 (Cat # MAB1155) was diluted in 1% BSA/TBST and incubated overnight at 4 °C. After washing 3 x 5 min with TBST the blots were incubated with goat anti-mouse or goat anti-rabbit secondary antibody for 1 hr at room temperature. The blots were again washed 3 x 5 min with TBST and developed using ECL reagent. Data is kindly provided by Dr. Weis of Cheresh Lab, UCSD.
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Specification
Product Description
Mouse monoclonal antibody raised against full length recombinant SRC.
Immunogen
Recombinant protein corresponding to full length human SRC.
Host
Mouse
Reactivity
Human, Mouse
Form
Liquid
Purification
Protein G purification
Isotype
IgG1
Recommend Usage
Western Blot (1:100-500)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
The SRC monoclonal antibody, clone 17AT28 (Cat # MAB1155) is used in Western blot to detect SRC in Jurkat cell lysate.Western Blot (Transfected lysate)
FG Pancreatic Carcinoma Cell Lines stably expressing vector along (FG-V) the beta3 integrin subunit (FG-beta3) or a beta3 truncation mutant (FG-759x) . SRC monoclonal antibody, clone 17AT28 (Cat # MAB1155) was diluted in 1% BSA/TBST and incubated overnight at 4 °C. After washing 3 x 5 min with TBST the blots were incubated with goat anti-mouse or goat anti-rabbit secondary antibody for 1 hr at room temperature. The blots were again washed 3 x 5 min with TBST and developed using ECL reagent. Data is kindly provided by Dr. Weis of Cheresh Lab, UCSD. -
Gene Info — SRC
Entrez GeneID
6714Protein Accession#
P12931Gene Name
SRC
Gene Alias
ASV, SRC1, c-SRC, p60-Src
Gene Description
v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian)
Omim ID
190090Gene Ontology
HyperlinkGene Summary
This gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq
Other Designations
OTTHUMP00000174476|OTTHUMP00000174477|proto-oncogene tyrosine-protein kinase SRC|protooncogene SRC, Rous sarcoma|tyrosine kinase pp60c-src|tyrosine-protein kinase SRC-1
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Interactome
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Pathway
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Disease
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Publication Reference
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Microexon-based regulation of ITSN1 and Src SH3 domains specificity relies on introduction of charged amino acids into the interaction interface.
Dergai M, Tsyba L, Dergai O, Zlatskii I, Skrypkina I, Kovalenko V, Rynditch A.
Biochemical and Biophysical Research Communications 2010 Aug; 399(2):307.
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Expression of a Src family kinase in chronic myelogenous leukemia cells induces resistance to imatinib in a kinase-dependent manner.
Pene-Dumitrescu T, Smithgall TE.
The Journal of Biological Chemistry 2010 May; 285(28):21446.
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Human phosphatidylethanolamine-binding protein 4 promotes transactivation of estrogen receptor alpha (ERalpha) in human cancer cells by inhibiting proteasome-dependent ERalpha degradation via association with Src.
Liu H, Qiu J, Li N, Chen T, Cao X.
The Journal of Biological Chemistry 2010 May; 285(29):21934.
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Microexon-based regulation of ITSN1 and Src SH3 domains specificity relies on introduction of charged amino acids into the interaction interface.
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