The SRC monoclonal antibody, clone 17AT28 (Cat # MAB1155) is used in Western blot to detect SRC in Jurkat cell lysate.
Western Blot (Transfected lysate)
FG Pancreatic Carcinoma Cell Lines stably expressing vector along (FG-V) the beta3 integrin subunit (FG-beta3) or a beta3 truncation mutant (FG-759x) . SRC monoclonal antibody, clone 17AT28 (Cat # MAB1155) was diluted 1 : 500 in 1% BSA/TBST and incubated overnight at 4 °C. After washing 3 x 5 min with TBST the blots were incubated with 1 : 5000 goat anti-mouse or goat anti-rabbit secondary antibody for 1 hr at room temperature. The blots were again washed 3 x 5 min with TBST and developed using ECL reagent. Data is kindly provided by Dr. Weis of Cheresh Lab, UCSD.
This gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq