GAB1 monoclonal antibody, clone 1AT1979
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More Files
- More Functions
Western Blot
Rescue of the JNK pathway by expression of wild-type GAB1 in GAB1-/- cells. GAB1-/- cells were transiently transfected with 1, 2, or 4 ug of the expression construct for human GAB1 cDNA, using the GenePORTER 2 transfection reagent (Gene Therapy Systems Inc.). After incubation, the cells were irradiated with UV-B light at 400 J/m2 or left untreated. Exogenous expression of GAB1 in transfected cells was confirmed by anti-GAB1 immunoblot analysis with wild-type cells as positive control. +/+, wild-type cells; -/-, GAB1-/- cells. (Mol. Cell. Biol. 2004 Feb 15;24 (4) :1531-1539)
Immunoprecipitation
Wild-type (+/+) and Gab1-/- (-/-) cells were heat shocked (HS) at 42 °C for 1 h or irradiated with UV-B light (400 J/m2) and then incubated at 37°C for 1 h. Cell lysates were prepared and subjected to immunoprecipitation (IP) with an anti-JNK2 antibody. The immunoprecipitates were resolved by SDS- PAGE and immunoblotted (IB) with GAB1 monoclonal antibody, clone 1AT1979 (Cat # MAB1139) . Data courtesy of Dr. GS Feng at The Burnham Institute.
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Specification
Product Description
Mouse monoclonal antibody raised against full length recombinant GAB1.
Immunogen
Recombinant GST fusion protein corresponding to full length human GAB1.
Host
Mouse
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Isotype
IgG1
Recommend Usage
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Rescue of the JNK pathway by expression of wild-type GAB1 in GAB1-/- cells. GAB1-/- cells were transiently transfected with 1, 2, or 4 ug of the expression construct for human GAB1 cDNA, using the GenePORTER 2 transfection reagent (Gene Therapy Systems Inc.). After incubation, the cells were irradiated with UV-B light at 400 J/m2 or left untreated. Exogenous expression of GAB1 in transfected cells was confirmed by anti-GAB1 immunoblot analysis with wild-type cells as positive control. +/+, wild-type cells; -/-, GAB1-/- cells. (Mol. Cell. Biol. 2004 Feb 15;24 (4) :1531-1539)Immunoprecipitation
Wild-type (+/+) and Gab1-/- (-/-) cells were heat shocked (HS) at 42 °C for 1 h or irradiated with UV-B light (400 J/m2) and then incubated at 37°C for 1 h. Cell lysates were prepared and subjected to immunoprecipitation (IP) with an anti-JNK2 antibody. The immunoprecipitates were resolved by SDS- PAGE and immunoblotted (IB) with GAB1 monoclonal antibody, clone 1AT1979 (Cat # MAB1139) . Data courtesy of Dr. GS Feng at The Burnham Institute. -
Gene Info — GAB1
Entrez GeneID
2549Protein Accession#
NP_997006;Q13480Gene Name
GAB1
Gene Alias
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Gene Description
GRB2-associated binding protein 1
Omim ID
604439Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the IRS1-like multisubstrate docking protein family. It is an important mediator of branching tubulogenesis and plays a central role in cellular growth response, transformation and apoptosis. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
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Interactome
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Pathway
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Disease
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Publication Reference
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Grb2-associated binder 1 (Gab1) genetic polymorphism, Helicobacter pylori infection, and chronic atrophic gastritis among older adults from Germany.
Gao L, Weck MN, Nieters A, Brenner H.
Molecular Carcinogenesis 2010 Oct; 49(10):869.
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An approach based on a genome-wide association study reveals candidate loci for narcolepsy.
Shimada M, Miyagawa T, Kawashima M, Tanaka S, Honda Y, Honda M, Tokunaga K.
Human Genetics 2010 Oct; 128(4):433.
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Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study.
Bailey SD, Xie C, Do R, Montpetit A, Diaz R, Mohan V, Keavney B, Yusuf S, Gerstein HC, Engert JC, Anand S.
Diabetes Care 2010 Jul; 33(10):2250.
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Grb2-associated binder 1 (Gab1) genetic polymorphism, Helicobacter pylori infection, and chronic atrophic gastritis among older adults from Germany.
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