KRT8 monoclonal antibody, clone H1
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More Files
- More Functions
Immunohistochemistry
Immunohistochemical staining of human colon with KRT8 monoclonal antibody, clone H1 (Cat # MAB11343) at 1-2 ug/mL, for 30 min at RT.
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Specification
Product Description
Mouse monoclonal antibody raised against KRT8.
Immunogen
Cytoskeleton preparation containing cytokeratin 8.
Host
Mouse
Reactivity
Human, Rat, Zebra fish
Form
Liquid
Purification
Protein A/G affinity chromatography
Isotype
IgG1
Recommend Usage
Flow Cytometry (0.5-1 ug/million cells)
Immunofluorescence (1-2 ug/mL)
Immunohistochemistry (1-2 ug/mL for 30 min at RT)
Immunoprecipitation (1-2 ug/500 ug protein)
Western Blot (0.25-0.5 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (0.05% BSA, 0.05% sodium azide)
Storage Instruction
Store at 4°C.
Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Immunohistochemistry
Immunohistochemical staining of human colon with KRT8 monoclonal antibody, clone H1 (Cat # MAB11343) at 1-2 ug/mL, for 30 min at RT.Immunofluorescence
Immunoprecipitation
Flow Cytometry
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Gene Info — KRT8
Entrez GeneID
3856Gene Name
KRT8
Gene Alias
CARD2, CK8, CYK8, K2C8, K8, KO
Gene Description
keratin 8
Gene Ontology
HyperlinkGene Summary
This gene is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis. [provided by RefSeq
Other Designations
cytokeratin 8|keratin, type II cytoskeletal 8
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Interactome
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Disease
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