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- Home > Products > Secondary/Tag Ab > Tag Ab > GST Tag > GST monoclonal antibody, clone 1E5
GST monoclonal antibody, clone 1E5
- Catalog # : MAB1132
- Visit Frequency :
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- 100 uL
- USD $ 319
- order now, ship in 5 days
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Email : sales@abnova.com
Phone : +1-909-992-0619 (new)
Fax : +1-909-992-3401- Products will be shipped directly to the customer.
- Specification
- Product Description:
- Mouse monoclonal antibody raised against full length recombinant GST.
- Immunogen:
- Recombinant protein corresponding to full length GST .
- Host:
- Mouse
- Reactivity:
- Human
- Form:
- Liquid
- Isotype:
- IgG2b, kappa
- Recommend Usage:
- Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.
- Storage Buffer:
- In PBS, pH 7.4 (0.1% sodium azide)
- Storage Instruction:
- Store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Publication Reference
- 1.
- Conventional immunoassays underestimate anti-GST antibody titre.
Cartwright GA, Rothel JS, Lightowlers MW.J Immunol Methods. 1995 Feb 13;179(1):31-5.
- 2.
- A non-radioactive assay for the detection and quantitation of a DNA binding protein.
Hibma MH, Ely SJ, Crawford L.Nucleic Acids Res. 1994 Sep 11;22(18):3806-7.
- 3.
- Mr 26,000 antigen of Schistosoma japonicum recognized by resistant WEHI 129/J mice is a parasite glutathione S-transferase.
Smith DB, Davern KM, Board PG, Tiu WU, Garcia EG, Mitchell GF.Proc Natl Acad Sci U S A. 1986 Nov;83(22):8703-7.
- Applications
- Western Blot (Recombinant protein)
- Western blot analysis of recombinant GST (28 kDa) and GST - fusion protein (61 kDa) were resolved by SDS - PAGE , transferred to PVDF membrane and probed with GST monoclonal antibody, clone 1E5 (1 : 1000) (Cat # MAB1132) . Proteins were visualized using a goat anti - mouse secondary antibody conjugated to HRP and an ECL detection system. Arrows indicate GST and GST - fusion proteins (20 ng) .
