GST monoclonal antibody, clone 1E5
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More Files
- More Functions
Western Blot (Recombinant protein)
Western blot analysis of recombinant protein GST (50 ng, 28kDa) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with GST monoclonal antibody, clone 1E5 (1:1000).
Immunofluorescence
Immunofluorescence analysis of HeLa cells. The cell was stained with GST monoclonal antibody, clone 1E5 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant human GST.
Immunogen
Recombinant protein corresponding to human GST.
Host
Mouse
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Isotype
IgG2b, kappa
Recommend Usage
ELISA
Immunocytochemistry (1:100)
Immunofluorescence (1:100)
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (10% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Recombinant protein)
Western blot analysis of recombinant protein GST (50 ng, 28kDa) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with GST monoclonal antibody, clone 1E5 (1:1000).Immunocytochemistry
Immunofluorescence
Immunofluorescence analysis of HeLa cells. The cell was stained with GST monoclonal antibody, clone 1E5 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).Enzyme-linked Immunoabsorbent Assay
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Publication Reference
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A Novel Function of Novobiocin: Disrupting the Interaction of HIF 1α and p300/CBP through Direct Binding to the HIF1α C-Terminal Activation Domain.
Wu D, Zhang R, Zhao R, Chen G, Cai Y, Jin J.
PLoS One 2013 May; 8(5):e62014.
Application：WB-Ce, Human, HeLa cells.
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Conventional immunoassays underestimate anti-GST antibody titre.
Cartwright GA, Rothel JS, Lightowlers MW.
Journal of Immunological Methods 1995 Feb; 179(1):31.
Application：C-ELISA, ELISA, N/A, Recombinant protein.
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A non-radioactive assay for the detection and quantitation of a DNA binding protein.
Hibma MH, Ely SJ, Crawford L.
Nucleic Acids Research 1994 Sep; 22(18):3806.
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Mr 26,000 antigen of Schistosoma japonicum recognized by resistant WEHI 129/J mice is a parasite glutathione S-transferase.
Smith DB, Davern KM, Board PG, Tiu WU, Garcia EG, Mitchell GF.
PNAS 1986 Nov; 83(22):8703.
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A Novel Function of Novobiocin: Disrupting the Interaction of HIF 1α and p300/CBP through Direct Binding to the HIF1α C-Terminal Activation Domain.
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