EPCAM monoclonal antibody, clone VU1D9
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More Files
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) analysis of human colon cancer with EPCAM monoclonal antibody, clone VU1D9 (Cat # MAB11316) at 1:100 using peroxidase-conjugate and DAB chromogen. Immunohistochemical staining of formalin/paraffin tissues is enhanced by unmasking of the target epitope with trypsin at 1 mg/ml PBS for 15 min at 37°C.
Flow Cytometry
Flow cytometric analysis of HT-29 cells using EpCAM monoclonal antibody, clone VU1D9 (Cat #MAB11316) (red) and isotype control (green) at 0.2 ug/106 cells.
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Specification
Product Description
Mouse monoclonal antibody raised against EpCAM (CD326).
Immunogen
The NC1-H69 human small cell lung carcinoma line.
Host
Mouse
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Isotype
IgG1, kappa
Recommend Usage
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:100-1:200)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% BSA, 0.05% sodium azide)
Storage Instruction
Store at 4°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) analysis of human colon cancer with EPCAM monoclonal antibody, clone VU1D9 (Cat # MAB11316) at 1:100 using peroxidase-conjugate and DAB chromogen. Immunohistochemical staining of formalin/paraffin tissues is enhanced by unmasking of the target epitope with trypsin at 1 mg/ml PBS for 15 min at 37°C.Flow Cytometry
Flow cytometric analysis of HT-29 cells using EpCAM monoclonal antibody, clone VU1D9 (Cat #MAB11316) (red) and isotype control (green) at 0.2 ug/106 cells. -
Gene Info — EPCAM
Entrez GeneID
4072Gene Name
EPCAM
Gene Alias
17-1A, 323/A3, CD326, CO-17A, CO17-1A, EGP, EGP-2, EGP34, EGP40, ESA, Ep-CAM, GA733-2, HEA125, KS1/4, KSA, M4S1, MH99, MIC18, MK-1, MOC31, TACST-1, TACSTD1, TROP1, hEGP-2
Gene Description
epithelial cell adhesion molecule
Omim ID
185535Gene Ontology
HyperlinkGene Summary
This gene encodes a carcinoma-associated antigen and is a member of a family that includes at least two type I membrane proteins. This antigen is expressed on most normal epithelial cells and gastrointestinal carcinomas and functions as a homotypic calcium-independent cell adhesion molecule. The antigen is being used as a target for immunotherapy treatment of human carcinomas. Mutations in this gene result in congenital tufting enteropathy. [provided by RefSeq
Other Designations
adenocarcinoma-associated antigen|carcinoma-associated antigen GA733-2|human epithelial glycoprotein-2|membrane component, chromosome 4, surface marker (35kD glycoprotein)|tumor-associated calcium signal transducer 1
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Interactome
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Disease
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