KRT18 monoclonal antibody, clone DA7
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Specification
Product Description
Mouse monoclonal antibody raised against KRT18.
Immunogen
Human breast cancer PMC 42 cells.
Host
Mouse
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Isotype
IgG1, kappa
Recommend Usage
Western Blot (1-3 ug/ml)
Immunocytochemistry/Immunofluorescence (1:10 - 1:2000)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:200 - 1:400)
Immunoprecipitation (1:10 - 1:500)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% BSA, 0.05% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of HeLa cell lysate using KRT18 monoclonal antibody, clone DA7 (Cat # MAB11308) at 1 ug/ml.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) analysis of human colon cancer with KRT18 monoclonal antibody, clone DA7 (Cat # MAB11308) at 1:200 using peroxidase-conjugate and DAB chromogen (Note: cytoplasmic staining of tumor cells).Immunocytochemistry
Immunofluorescence
Immunoprecipitation
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Gene Info — KRT18
Entrez GeneID
3875Gene Name
KRT18
Gene Alias
CYK18, K18
Gene Description
keratin 18
Gene Ontology
HyperlinkGene Summary
KRT18 encodes the type I intermediate filament chain keratin 18. Keratin 18, together with its filament partner keratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq
Other Designations
cell proliferation-inducing protein 46|cytokeratin 18
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Interactome
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Disease
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