NCAM1 monoclonal antibody, clone 123C3.D5
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More Files
- More Functions
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) analysis of human colon ganglion cells with NCAM1 monoclonal antibody, clone 123C3.D5 (Cat # MAB11301) at 1:100 using peroxidase-conjugate and DAB chromogen. Staining of formalin/paraffin tissues REQUIRES boiling tissue sections in 10mM citrate buffer, pH 6.0.
Flow Cytometry
Flow cytometric analysis of human monocytes using NCAM1 monoclonal antibody, clone 123C3.D5 (Cat # MAB11301) at 1 ug/106 cells.
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Specification
Product Description
Mouse monoclonal antibody raised against NCAM1.
Immunogen
A membrane preparation of a small cell lung carcinoma.
Host
Mouse
Reactivity
Human
Specificity
This antibody recognizes two proteins (185 kDa & 145 kDa), identified as two isoforms of neural cell adhesion molecule (NCAM1/CD56).
Form
Liquid
Purification
Protein G purification
Isotype
IgG1, kappa
Recommend Usage
Western Blot (1:100-1:2000)
Flow Cytometry (1-2 ug/106 cells)
Immunocytochemistry/Immunofluorescence (1:10-1:2000)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:100-1:200)
Immunoprecipitation (1:10-1:500)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% BSA, 0.05% sodium azide)
Storage Instruction
Store at 4°C.
Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) analysis of human colon ganglion cells with NCAM1 monoclonal antibody, clone 123C3.D5 (Cat # MAB11301) at 1:100 using peroxidase-conjugate and DAB chromogen. Staining of formalin/paraffin tissues REQUIRES boiling tissue sections in 10mM citrate buffer, pH 6.0.Immunocytochemistry
Immunofluorescence
Immunoprecipitation
Flow Cytometry
Flow cytometric analysis of human monocytes using NCAM1 monoclonal antibody, clone 123C3.D5 (Cat # MAB11301) at 1 ug/106 cells. -
Gene Info — NCAM1
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Interactome
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Pathway
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Disease
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