BCL2 monoclonal antibody, clone 8C8
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of cell lysates by BCL2 monoclonal antibody, clone 8C8 (Cat # MAB11265) at 2 ug/ml. Lane1 : HeLa. Lane2 : A549. Lane3 : MCF7.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human tonsil with BCL2 monoclonal antibody, clone 8C8 (Cat # MAB11265) at 1:200 dilution.
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Specification
Product Description
Mouse monoclonal antibody raised against synthetic peptide of BCL2.
Immunogen
A synthetic peptide corresponding to amino acids 41-54 of human BCL2.
Sequence
GAAPAPGIFSSQPG
Host
Mouse
Reactivity
Human, Monkey
Form
Liquid
Purification
Protein G purification
Isotype
IgG1 Kappa
Recommend Usage
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:200-1:400)
Western Blot (1-3 ug/ml)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% BSA, 0.05% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of cell lysates by BCL2 monoclonal antibody, clone 8C8 (Cat # MAB11265) at 2 ug/ml. Lane1 : HeLa. Lane2 : A549. Lane3 : MCF7.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human tonsil with BCL2 monoclonal antibody, clone 8C8 (Cat # MAB11265) at 1:200 dilution.Immunocytochemistry
Immunofluorescence
Flow Cytometry
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Gene Info — BCL2
Entrez GeneID
596Protein Accession#
NP_000624Gene Name
BCL2
Gene Alias
Bcl-2
Gene Description
B-cell CLL/lymphoma 2
Omim ID
151430Gene Ontology
HyperlinkGene Summary
This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Two transcript variants, produced by alternate splicing, differ in their C-terminal ends. [provided by RefSeq
Other Designations
B-cell lymphoma protein 2|OTTHUMP00000163680
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Interactome
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Pathway
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Disease
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