EPCAM monoclonal antibody
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More Files
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Western Blot (Cell lysate)
Western blot analysis of EPCAM monoclonal antibody (Cat # MAB11144) at 1:1000 dilution.
Lane 1: HepG-2 whole cell lysate 40 ug/lane.
Lane 2: A-431 whole cell lysate 40 ug/lane.
Lane 3: MCF-7 whole cell lysate 40 ug/lane.
Lane 4: HCT116 whole cell lysate 40 ug/lane.
Predicted band size: 39 KDa. Observed band size: 39 KDa. Addition band size: 34 KDa.
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant protein of EPCAM.
Immunogen
Recombinant protein corresponding to N-terminus of human EPCAM.
Host
Mouse
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Recommend Usage
ELISA (1:5000-1:20000)
Western Blot (1:100-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of EPCAM monoclonal antibody (Cat # MAB11144) at 1:1000 dilution.
Lane 1: HepG-2 whole cell lysate 40 ug/lane.
Lane 2: A-431 whole cell lysate 40 ug/lane.
Lane 3: MCF-7 whole cell lysate 40 ug/lane.
Lane 4: HCT116 whole cell lysate 40 ug/lane.
Predicted band size: 39 KDa. Observed band size: 39 KDa. Addition band size: 34 KDa.Enzyme-linked Immunoabsorbent Assay
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Gene Info — EPCAM
Entrez GeneID
4072Protein Accession#
P16422;NM_002354.1Gene Name
EPCAM
Gene Alias
17-1A, 323/A3, CD326, CO-17A, CO17-1A, EGP, EGP-2, EGP34, EGP40, ESA, Ep-CAM, GA733-2, HEA125, KS1/4, KSA, M4S1, MH99, MIC18, MK-1, MOC31, TACST-1, TACSTD1, TROP1, hEGP-2
Gene Description
epithelial cell adhesion molecule
Omim ID
185535Gene Ontology
HyperlinkGene Summary
This gene encodes a carcinoma-associated antigen and is a member of a family that includes at least two type I membrane proteins. This antigen is expressed on most normal epithelial cells and gastrointestinal carcinomas and functions as a homotypic calcium-independent cell adhesion molecule. The antigen is being used as a target for immunotherapy treatment of human carcinomas. Mutations in this gene result in congenital tufting enteropathy. [provided by RefSeq
Other Designations
adenocarcinoma-associated antigen|carcinoma-associated antigen GA733-2|human epithelial glycoprotein-2|membrane component, chromosome 4, surface marker (35kD glycoprotein)|tumor-associated calcium signal transducer 1
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Interactome
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Disease
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