HSP90AA1 monoclonal antibody, clone 4F10
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More Files
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Western Blot (Cell lysate)
Western blot analysis of cell lysates of HeLa (each 20 ug) was resolved by SDS - PAGE , transferred to PVDF membrane and probed with HSP90AA1 monoclonal antibody , clone 4F10 (1 : 1000) (Cat # MAB1092). Proteins were visualized using a goat anti - mouse secondary antibody conjugated to HRP and an ECL detection system.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry of human urothelium were incubated with HSP90AA1 monoclonal antibody, clone 4F10 (1:100) for 2 hours at room temperature. Antigen retrieval was performed in 0.1 M sodium citrate buffer and detected using Diaminobenzidine (DAB).
Immunofluorescence
Immunofluorescence analysis of HeLa cells. The cell was stained with HSP90AA1 monoclonal antibody, clone 4F10 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
Flow Cytometry
Flow cytometric analysis of HepG2 cell line, staining at 2-5 ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).
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Specification
Product Description
Mouse monoclonal antibody raised against full length recombinant HSP90AA1.
Immunogen
Recombinant protein corresponding to full length human HSP90AA1.
Host
Mouse
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Isotype
IgG2b, kappa
Recommend Usage
ELISA
Flow Cytometry
Immunocytochemistry
Immunofluorescence
Immunohistochemistry
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (10% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of cell lysates of HeLa (each 20 ug) was resolved by SDS - PAGE , transferred to PVDF membrane and probed with HSP90AA1 monoclonal antibody , clone 4F10 (1 : 1000) (Cat # MAB1092). Proteins were visualized using a goat anti - mouse secondary antibody conjugated to HRP and an ECL detection system.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry of human urothelium were incubated with HSP90AA1 monoclonal antibody, clone 4F10 (1:100) for 2 hours at room temperature. Antigen retrieval was performed in 0.1 M sodium citrate buffer and detected using Diaminobenzidine (DAB).Immunocytochemistry
Immunofluorescence
Immunofluorescence analysis of HeLa cells. The cell was stained with HSP90AA1 monoclonal antibody, clone 4F10 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Flow cytometric analysis of HepG2 cell line, staining at 2-5 ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line). -
Gene Info — HSP90AA1
Entrez GeneID
3320GeneBank Accession#
NM_005348Protein Accession#
NP_005339Gene Name
HSP90AA1
Gene Alias
FLJ31884, HSP86, HSP89A, HSP90A, HSP90N, HSPC1, HSPCA, HSPCAL1, HSPCAL4, HSPN, Hsp89, Hsp90, LAP2
Gene Description
heat shock protein 90kDa alpha (cytosolic), class A member 1
Omim ID
140571Gene Ontology
HyperlinkGene Summary
HSP90 proteins are highly conserved molecular chaperones that have key roles in signal transduction, protein folding, protein degradation, and morphologic evolution. HSP90 proteins normally associate with other cochaperones and play important roles in folding newly synthesized proteins or stabilizing and refolding denatured proteins after stress. There are 2 major cytosolic HSP90 proteins, HSP90AA1, an inducible form, and HSP90AB1 (MIM 140572), a constitutive form. Other HSP90 proteins are found in endoplasmic reticulum (HSP90B1; MIM 191175) and mitochondria (TRAP1; MIM 606219) (Chen et al., 2005 [PubMed 16269234]).[supplied by OMIM
Other Designations
heat shock 90kD protein 1, alpha|heat shock 90kD protein 1, alpha-like 4|heat shock 90kD protein, alpha-like 4|heat shock 90kDa protein 1, alpha
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Interactome
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Pathway
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Disease
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Publication Reference
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pH-Promoted Release of a Novel Anti-Tumour Peptide by "Stealth" Liposomes: Effect of Nanocarriers on the Drug Activity in Cis-Platinum Resistant Cancer Cells.
Sacchetti F, Marverti G, D'Arca D, Severi L, Maretti E, Iannuccelli V, Pacifico S, Ponterini G, Costi MP, Leo E.
Pharmaceutical Research 2018 Sep; 35(11):206.
Application：WB-Ce, Human, C13* cells.
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Mass spectrometric/bioinformatic identification of a protein subset that characterizes the cellular activity of anticancer peptides.
Genovese F, Gualandi A, Taddia L, Marverti G, Pirondi S, Marraccini C, Perco P, Pelà M, Guerrini R, Amoroso MR, Esposito F, Martello A, Ponterini G, D'Arca D, Costi MP.
The Journal of Proteome Research 2014 Nov; 13(11):5250.
Application：WB-Ce, Human, CSD-OC, A2780/CP, IGROV-1 cells.
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Increased proliferation of B cells and auto-immunity in mice lacking protein kinase Cdelta.
Miyamoto A, Nakayama K, Imaki H, Hirose S, Jiang Y, Abe M, Tsukiyama T, Nagahama H, Ohno S, Hatakeyama S, Nakayama KI.
Nature 2002 Apr; 416(6883):865.
Application：WB-Ti, Mouse, Brain.
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Heat shock protein 90 mediates the balance of nitric oxide and superoxide anion from endothelial nitric-oxide synthase.
Pritchard KA Jr, Ackerman AW, Gross ER, Stepp DW, Shi Y, Fontana JT, Baker JE, Sessa WC.
The Journal of Biological Chemistry 2001 May; 276(21):17621.
Application：WB-Ce, Bovine, Bovine coronary endothelial cells.
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Quantitation and intracellular localization of the 85K heat shock protein by using monoclonal and polyclonal antibodies.
Lai BT, Chin NW, Stanek AE, Keh W, Lanks KW.
Molecular and Cellular Biology 1984 Dec; 4(12):2802.
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pH-Promoted Release of a Novel Anti-Tumour Peptide by "Stealth" Liposomes: Effect of Nanocarriers on the Drug Activity in Cis-Platinum Resistant Cancer Cells.
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