HSPB1 monoclonal antibody, clone 2A5
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of Lane 1: A431 cell lysate, Lane 2: HepG2 cell lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry of human colon cancer tissue were incubated with HSPB1 monoclonal antibody, clone 2A5 (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB).
Immunofluorescence
Immunofluorescence analysis of HeLa cells. The cell was stained with HSPB1 monoclonal antibody, clone 2A5 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
Flow Cytometry
Flow cytometric analysis of A-549 cell line, staining at 2-5 ug for 1x106cells. The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate.
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Specification
Product Description
Mouse monoclonal antibody raised against full length recombinant HSPB1.
Immunogen
Recombinant protein corresponding to full length human HSPB1.
Host
Mouse
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Isotype
IgG1, kappa
Recommend Usage
ELISA
Flow Cytometry
Immunocytochemistry
Immunofluorescence
Immunohistochemistry
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (10% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane 1: A431 cell lysate, Lane 2: HepG2 cell lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry of human colon cancer tissue were incubated with HSPB1 monoclonal antibody, clone 2A5 (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB).Immunocytochemistry
Immunofluorescence
Immunofluorescence analysis of HeLa cells. The cell was stained with HSPB1 monoclonal antibody, clone 2A5 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Flow cytometric analysis of A-549 cell line, staining at 2-5 ug for 1x106cells. The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. -
Gene Info — HSPB1
Entrez GeneID
3315GeneBank Accession#
NM_001540Protein Accession#
NP_001531Gene Name
HSPB1
Gene Alias
CMT2F, DKFZp586P1322, HMN2B, HS.76067, HSP27, HSP28, Hsp25, SRP27
Gene Description
heat shock 27kDa protein 1
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is induced by environmental stress and developmental changes. The encoded protein is involved in stress resistance and actin organization and translocates from the cytoplasm to the nucleus upon stress induction. Defects in this gene are a cause of Charcot-Marie-Tooth disease type 2F (CMT2F) and distal hereditary motor neuropathy (dHMN). [provided by RefSeq
Other Designations
OTTHUMP00000024846|estrogen-regulated 24 kDa protein|heat shock 27kD protein 1|heat shock protein beta-1|stress-responsive protein 27
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Interactome
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Pathway
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Disease
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Publication Reference
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Stress protection by a fluorescent Hsp27 chimera that is independent of nuclear translocation or multimeric dissociation.
Borrelli MJ, Bernock LJ, Landry J, Spitz DR, Weber LA, Hickey E, Freeman ML, Corry PM.
Cell Stress & Chaperones 2002 Jul; 7(3):281.
Application：IF, WB-Tr, Human, A-549 cells.
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Heat shock protein 27 is a substrate of cGMP-dependent protein kinase in intact human platelets: phosphorylation-induced actin polymerization caused by HSP27 mutants.
Butt E, Immler D, Meyer HE, Kotlyarov A, Laass K, Gaestel M.
The Journal of Biological Chemistry 2001 Mar; 276(10):7108.
Application：WB-Ce, Human, Platelets.
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Inhibition of Daxx-mediated apoptosis by heat shock protein 27.
Charette SJ, Lavoie JN, Lambert H, Landry J.
Molecular and Cellular Biology 2000 Oct; 20(20):7602.
Application：WB-Tr, Human, HEK 293 cells.
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Stress protection by a fluorescent Hsp27 chimera that is independent of nuclear translocation or multimeric dissociation.
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