CRP monoclonal antibody, clone 5A9
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of cell lysates (each 40 ug). Lane 1 : NIH/3T3. Lane 2 : HepG2. Lane 3 : Jurkat. Lane 4 : HeLa. Lane 5 : SH - SY5Y. To probed CRP monoclonal antibody , clone 5A9 (1 : 1000) (Cat # MAB1089). Proteins were visualized using a goat anti - mouse secondary antibody conjugated to HRP and an ECL detection system.
Immunocytochemistry
Immunofluorescence analysis of HepG2 cells. The cell was stained with CRP monoclonal antibody, clone 5A9 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
Flow Cytometry
Flow cytometric analysis of HepG2 cell line, staining at 2-5 ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant CRP.
Immunogen
Recombinant protein corresponding to amino acids 19-224 of human CRP.
Host
Mouse
Reactivity
Human, Mouse
Form
Liquid
Purification
Protein G purification
Isotype
IgG2a, kappa
Recommend Usage
ELISA
Flow Cytometry
Immunocytochemistry
Immunofluorescence
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (10% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of cell lysates (each 40 ug). Lane 1 : NIH/3T3. Lane 2 : HepG2. Lane 3 : Jurkat. Lane 4 : HeLa. Lane 5 : SH - SY5Y. To probed CRP monoclonal antibody , clone 5A9 (1 : 1000) (Cat # MAB1089). Proteins were visualized using a goat anti - mouse secondary antibody conjugated to HRP and an ECL detection system.Immunocytochemistry
Immunofluorescence analysis of HepG2 cells. The cell was stained with CRP monoclonal antibody, clone 5A9 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).Immunofluorescence
Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Flow cytometric analysis of HepG2 cell line, staining at 2-5 ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line). -
Gene Info — CRP
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Interactome
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Disease
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Publication Reference
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C-reactive protein in hypertension: clinical significance and predictive value.
Schillaci G, Pirro M.
Nutrition, Metabolism, and Cardiovascular Diseases 2006 Oct; 16(7):500.
Application：ELISA, Human, Human serum.
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Human C-reactive protein is protective against fatal Salmonella enterica serovar typhimurium infection in transgenic mice.
Szalai AJ, VanCott JL, McGhee JR, Volanakis JE, Benjamin WH Jr.
Infection and Immunity 2000 Oct; 68(10):5652.
Application：ELISA, Human, Serum.
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C-reactive protein in hypertension: clinical significance and predictive value.
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