Mouse monoclonal antibody raised against partial recombinant CASP2.
Immunogen:
Recombinant protein corresponding to amino acids 32-169 of human CASP2.
Host:
Mouse
Reactivity:
Human
Form:
Liquid
Isotype:
IgG2b, kappa
Recommend Usage:
Western Blot (1:500-1:1000) The optimal working dilution should be determined by the end user.
Storage Buffer:
In PBS, pH 7.4 (0.1% sodium azide)
Storage Instruction:
Store at -20°C. Aliquot to avoid repeated freezing and thawing.
Note:
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Applications
Western Blot (Cell lysate)
Western blot analysis of cell lysates (each 40 ug). Lane 1 : Ramos. Lane 2 : Jurkat. To probed with CASP2 monoclonal antibody , clone 1C10 (1 : 500) (Cat # MAB1084). Proteins were visualized using a goat anti - mouse secondary antibody conjugated to HRP and a ECL detection system.
This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. The proteolytic cleavage of this protein is induced by a variety of apoptotic stimuli. Alternative splicing of this gene results in multiple transcript variants that encode different isoforms. [provided by RefSeq