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Last updated: 2014/9/1
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NFKB1 monoclonal antibody, clone 5D10

  • Catalog # : MAB10829
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against partial recombinant NFKB1.
  • Immunogen:
  • Recombinant protein corresponding to human NFKB1.
  • Host:
  • Mouse
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Isotype:
  • IgG2a
  • Recommend Usage:
  • ELISA (1:10000)
    Western Blot (1:500-1:2000)
    Immunohistochemistry (1:200-1:1000)
    Flow cytometry (1:200-1:400)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In ascites (0.03% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis using NFKB1 monoclonal antibody, clone 5D10 (Cat # MAB10829) against K-562 (1), Jurkat (2), A-431 (3), HeLa (4), THP-1 (5) and MCF-7 (6) cell lysate.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical analysis of paraffin-embedded human prostate tissues (A) and bladder cancer tissues (B) using NFKB1 monoclonal antibody, clone 5D10 (Cat # MAB10829) with DAB staining.
  • ELISA
  • ELISA
  • ELISA measurement of NFKB1 monoclonal antibody, clone 5D10 (Cat # MAB10829).
  • Flow Cytometry
  • Flow Cytometry
  • Flow cytometric analysis of MCF-7 cells using NFKB1 monoclonal antibody, clone 5D10 (Cat # MAB10829) (green) and negative control (purple).
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 4790
  • Gene Name:
  • NFKB1
  • Gene Alias:
  • DKFZp686C01211,EBP-1,KBF1,MGC54151,NF-kappa-B,NFKB-p105,NFKB-p50,p105,p50
  • Gene Description:
  • nuclear factor of kappa light polypeptide gene enhancer in B-cells 1
  • Gene Summary:
  • This gene encodes a 105 kD protein which can undergo cotranslational processing by the 26S proteasome to produce a 50 kD protein. The 105 kD protein is a Rel protein-specific transcription inhibitor and the 50 kD protein is a DNA binding subunit of the NF-kappa-B (NFKB) protein complex. NFKB is a transcription regulator that is activated by various intra- and extra-cellular stimuli such as cytokines, oxidant-free radicals, ultraviolet irradiation, and bacterial or viral products. Activated NFKB translocates into the nucleus and stimulates the expression of genes involved in a wide variety of biological functions. Inappropriate activation of NFKB has been associated with a number of inflammatory diseases while persistent inhibition of NFKB leads to inappropriate immune cell development or delayed cell growth. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
  • Other Designations:
  • DNA binding factor KBF1,NF-kappabeta,nuclear factor NF-kappa-B p50 subunit,nuclear factor kappa-B DNA binding subunit,nuclear factor kappa-B, subunit 1
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