KLHL13 monoclonal antibody, clone 8D1
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis using KLHL13 monoclonal antibody, clone 8D1 (Cat # MAB10720) against HeLa (1) and MCF-7 (2) cell lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human brain tissues (upper) and pancreas tissues (bottom) using KLHL13 monoclonal antibody, clone 8D1 (Cat # MAB10720) with DAB staining.
Immunofluorescence
Immunofluorescence analysis of NTERA-2 cells (upper) and U251 (bottom) cells using KLHL13 monoclonal antibody, clone 8D1 (Cat # MAB10720) (green) . Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Flow Cytometry
Flow cytometric analysis of 3T3/L1 cells using KLHL13 monoclonal antibody, clone 8D1 (Cat # MAB10720) (green) and negative control (purple).
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant KLHL13.
Immunogen
Recombinant protein corresponding to human KLHL13.
Host
Mouse
Theoretical MW (kDa)
74
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry (1:200-1:1000)
Immunofluorescence (1:200-1:1000)
Flow cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In ascites (0.03% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis using KLHL13 monoclonal antibody, clone 8D1 (Cat # MAB10720) against HeLa (1) and MCF-7 (2) cell lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human brain tissues (upper) and pancreas tissues (bottom) using KLHL13 monoclonal antibody, clone 8D1 (Cat # MAB10720) with DAB staining.Immunofluorescence
Immunofluorescence analysis of NTERA-2 cells (upper) and U251 (bottom) cells using KLHL13 monoclonal antibody, clone 8D1 (Cat # MAB10720) (green) . Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Flow cytometric analysis of 3T3/L1 cells using KLHL13 monoclonal antibody, clone 8D1 (Cat # MAB10720) (green) and negative control (purple). -
Gene Info — KLHL13
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Interactome
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Pathway
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