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Last updated: 2014/12/21
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KLHL13 monoclonal antibody, clone 8D1

  • Catalog # : MAB10720
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against partial recombinant KLHL13.
  • Immunogen:
  • Recombinant protein corresponding to human KLHL13.
  • Host:
  • Mouse
  • Theoretical MW (kDa):
  • 74
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Isotype:
  • IgG1
  • Recommend Usage:
  • ELISA (1:10000)
    Western Blot (1:500-1:2000)
    Immunohistochemistry (1:200-1:1000)
    Immunofluorescence (1:200-1:1000)
    Flow cytometry (1:200-1:400)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In ascites (0.03% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis using KLHL13 monoclonal antibody, clone 8D1 (Cat # MAB10720) against HeLa (1) and MCF-7 (2) cell lysate.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical analysis of paraffin-embedded human brain tissues (upper) and pancreas tissues (bottom) using KLHL13 monoclonal antibody, clone 8D1 (Cat # MAB10720) with DAB staining.
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescence analysis of NTERA-2 cells (upper) and U251 (bottom) cells using KLHL13 monoclonal antibody, clone 8D1 (Cat # MAB10720) (green) . Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
  • ELISA
  • Flow Cytometry
  • Flow Cytometry
  • Flow cytometric analysis of 3T3/L1 cells using KLHL13 monoclonal antibody, clone 8D1 (Cat # MAB10720) (green) and negative control (purple) .
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • ELISA
  • Gene Information
  • Gene Name:
  • KLHL13
  • Gene Alias:
  • BKLHD2,FLJ10262,KIAA1309,MGC74791
  • Gene Description:
  • kelch-like 13 (Drosophila)
  • Other Designations:
  • BTB and kelch domain containing 2,kelch-like 13
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