ADIPOQ monoclonal antibody, clone 5H7
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More Files
- More Functions
Western Blot (Transfected lysate)
Western blot analysis of Lane 1: Adiponectin collagen domain(15-107 a.a.) transfected 293T cell lysate; Lane 2: Adiponectin Full domain(15-244 a.a.) transfected 293T cell lysate; Lane 3: Adiponectin globular domain (108-244 a.a.) transfected 293T cell lysate.
Immunocytochemistry
Immunocytochemistry analysis of Hep3B cells. The cell was stained with ADIPOQ monoclonal antibody, clone 5H7 (Cat#MAB1072) (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant ADIPOQ.
Immunogen
Recombinant protein corresponding to amino acids 15-244 of human ADIPOQ.
Host
Mouse
Reactivity
Human, Mouse
Specificity
This antibody has the specificity against collagen-like domain of ADIPOQ.
Form
Liquid
Purification
Protein G purification
Isotype
IgG1, kappa
Recommend Usage
ELISA
Immunocytochemistry
Immunofluorescence
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (10% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis of Lane 1: Adiponectin collagen domain(15-107 a.a.) transfected 293T cell lysate; Lane 2: Adiponectin Full domain(15-244 a.a.) transfected 293T cell lysate; Lane 3: Adiponectin globular domain (108-244 a.a.) transfected 293T cell lysate.Immunocytochemistry
Immunocytochemistry analysis of Hep3B cells. The cell was stained with ADIPOQ monoclonal antibody, clone 5H7 (Cat#MAB1072) (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).Immunofluorescence
Enzyme-linked Immunoabsorbent Assay
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Gene Info — ADIPOQ
Entrez GeneID
9370GeneBank Accession#
NM_004797Protein Accession#
NP_004788Gene Name
ADIPOQ
Gene Alias
ACDC, ACRP30, ADPN, APM-1, APM1, GBP28, adiponectin
Gene Description
adiponectin, C1Q and collagen domain containing
Omim ID
605441Gene Ontology
HyperlinkGene Summary
This gene is expressed in adipose tissue exclusively. It encodes a protein with similarity to collagens X and VIII and complement factor C1q. The encoded protein circulates in the plasma and is involved with metabolic and hormonal processes. [provided by RefSeq
Other Designations
adipocyte, C1Q and collagen domain containing|adipocyte, C1Q and collagen domain-containing|adiponectin|adipose most abundant gene transcript 1|gelatin-binding protein 28
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Interactome
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Pathway
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Disease
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Publication Reference
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Adiponectin stimulates glucose utilization and fatty-acid oxidation by activating AMP-activated protein kinase.
Yamauchi T, Kamon J, Minokoshi Y, Ito Y, Waki H, Uchida S, Yamashita S, Noda M, Kita S, Ueki K, Eto K, Akanuma Y, Froguel P, Foufelle F, Ferre P, Carling D, Kimura S, Nagai R, Kahn BB, Kadowaki T.
Nature Medicine 2002 Nov; 8(11):1288.
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ACRP30/adiponectin: an adipokine regulating glucose and lipid metabolism.
Berg AH, Combs TP, Scherer PE.
Trends in Endocrinology and Metabolism 2002 Mar; 13(2):84.
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The adipocyte-secreted protein Acrp30 enhances hepatic insulin action.
Berg AH, Combs TP, Du X, Brownlee M, Scherer PE.
Nat Med 2001 Aug; 7(8):947.
Application：ELISA, WB, Mouse, Serum, Plasma.
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Adiponectin stimulates glucose utilization and fatty-acid oxidation by activating AMP-activated protein kinase.
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