CEBPB monoclonal antibody, clone 47A1
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of 293T cell lysate.
Immunocytochemistry
Immunocytochemistry analysis of HeLa cells. The cell was stained with CEBPB monoclonal antibody, clone 47A1 (Cat# MAB1069) (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant CEBPB.
Immunogen
Recombinant protein corresponding to amino acids 1-271 of human CEBPB.
Host
Mouse
Reactivity
Human, Mouse
Form
Liquid
Purification
Protein G purification
Isotype
IgG1, kappa
Recommend Usage
ELISA
Immunocytochemistry
Immunofluorescence
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (10% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of 293T cell lysate.Immunocytochemistry
Immunocytochemistry analysis of HeLa cells. The cell was stained with CEBPB monoclonal antibody, clone 47A1 (Cat# MAB1069) (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).Immunofluorescence
Enzyme-linked Immunoabsorbent Assay
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Gene Info — CEBPB
Entrez GeneID
1051GeneBank Accession#
NM_005194Protein Accession#
NP_005185Gene Name
CEBPB
Gene Alias
C/EBP-beta, CRP2, IL6DBP, LAP, MGC32080, NF-IL6, TCF5
Gene Description
CCAAT/enhancer binding protein (C/EBP), beta
Omim ID
189965Gene Ontology
HyperlinkGene Summary
The protein encoded by this intronless gene is a bZIP transcription factor which can bind as a homodimer to certain DNA regulatory regions. It can also form heterodimers with the related proteins CEBP-alpha, CEBP-delta, and CEBP-gamma. The encoded protein is important in the regulation of genes involved in immune and inflammatory responses and has been shown to bind to the IL-1 response element in the IL-6 gene, as well as to regulatory regions of several acute-phase and cytokine genes. In addition, the encoded protein can bind the promoter and upstream element and stimulate the expression of the collagen type I gene. [provided by RefSeq
Other Designations
CCAAT/enhancer binding protein beta|interleukin 6-dependent DNA-binding protein|liver-enriched transcriptional activator protein|nuclear factor of interleukin 6|transcription factor 5
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Interactome
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Disease
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Publication Reference
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Differential activation of a C/EBP beta isoform by a novel redox switch may confer the lipopolysaccharide-inducible expression of interleukin-6 gene.
Su WC, Chou HY, Chang CJ, Lee YM, Chen WH, Huang KH, Lee MY, Lee SC.
The Journal of Biological Chemistry 2003 Dec; 278(51):51150.
Application：WB-Tr, Human, HEK 293T cells.
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Regulation of CCAAT/enhancer-binding protein (C/EBP) activator proteins by heterodimerization with C/EBPgamma (Ig/EBP).
Parkin SE, Baer M, Copeland TD, Schwartz RC, Johnson PF.
The Journal of Biological Chemistry 2002 Jun; 277(26):23563.
Application：WB-Tr, Human, HepG2, L cells.
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Biological role of the CCAAT/enhancer-binding protein family of transcription factors.
Lekstrom-Himes J, Xanthopoulos KG.
The Journal of Biological Chemistry 1998 Oct; 273(44):28545.
Application：IHC, WB, Human, Mouse, Adipose tissues, Liver, Intestine, Lung, .
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Differential activation of a C/EBP beta isoform by a novel redox switch may confer the lipopolysaccharide-inducible expression of interleukin-6 gene.
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