GAPDH monoclonal antibody, clone 1A10
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis using GAPDH monoclonal antibody, clone 1A10 (Cat # MAB10662) against HeLa (1) , A-549 (2) , A-431 (3) , MCF-7 (4) , K-562 (5) , Jurkat (6) , HL-60 (7) , SKN-SH (8) and SK-BR-3 (9) cell lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma (upper) and kidney carcinoma (bottom) , showing cytoplasmic localization using GAPDH monoclonal antibody, clone 1A10 (Cat # MAB10662) with DAB staining.
Immunofluorescence
Confocal immunofluorescence analysis of methanol-fixed HepG2 (upper) and HeLa (bottom) cells using GAPDH monoclonal antibody, clone 1A10 (Cat # MAB10662) (green) , showing cytoplasmic localization. Blue: DRAQ5 fluorescent DNA dye.
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant GAPDH.
Immunogen
Recombinant protein corresponding to human GAPDH.
Host
Mouse
Theoretical MW (kDa)
37
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry (1:200-1:1000)
Immunofluorescence (1:200-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In ascites (0.03% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis using GAPDH monoclonal antibody, clone 1A10 (Cat # MAB10662) against HeLa (1) , A-549 (2) , A-431 (3) , MCF-7 (4) , K-562 (5) , Jurkat (6) , HL-60 (7) , SKN-SH (8) and SK-BR-3 (9) cell lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma (upper) and kidney carcinoma (bottom) , showing cytoplasmic localization using GAPDH monoclonal antibody, clone 1A10 (Cat # MAB10662) with DAB staining.Immunofluorescence
Confocal immunofluorescence analysis of methanol-fixed HepG2 (upper) and HeLa (bottom) cells using GAPDH monoclonal antibody, clone 1A10 (Cat # MAB10662) (green) , showing cytoplasmic localization. Blue: DRAQ5 fluorescent DNA dye.Enzyme-linked Immunoabsorbent Assay
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Gene Info — GAPDH
Entrez GeneID
2597Gene Name
GAPDH
Gene Alias
G3PD, GAPD, MGC88685
Gene Description
glyceraldehyde-3-phosphate dehydrogenase
Omim ID
138400Gene Ontology
HyperlinkGene Summary
The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains. Many pseudogenes similar to this locus are present in the human genome. [provided by RefSeq
Other Designations
OTTHUMP00000174431|OTTHUMP00000174432|aging-associated gene 9 protein|glyceraldehyde 3-phosphate dehydrogenase
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Interactome
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Pathway
- Biosynthesis of alkaloids derived from histidine and purine
- Biosynthesis of alkaloids derived from ornithine
- Biosynthesis of alkaloids derived from shikimate pathway
- Biosynthesis of alkaloids derived from terpenoid and polyketide
- Biosynthesis of phenylpropanoids
- Biosynthesis of plant hormones
- Biosynthesis of terpenoids and steroids
- Glycolysis / Gluconeogenesis
- Metabolic pathways
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Disease
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