Mouse monoclonal antibody raised against partial recombinant CTNNB1.
Immunogen:
Recombinant protein corresponding to human CTNNB1.
Host:
Mouse
Theoretical MW (kDa):
85
Reactivity:
Human
Form:
Liquid
Isotype:
IgG1
Recommend Usage:
ELISA (1:10000) Western Blot (1:500-1:2000) Immunohistochemistry (1:200-1:1000) Immunofluorescence (1:200-1:1000) Flow cytometry (1:200-1:400) The optimal working dilution should be determined by the end user.
Storage Buffer:
In ascites (0.03% sodium azide)
Storage Instruction:
Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing.
Note:
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Applications
Western Blot (Transfected lysate)
Western blot analysis using CTNNB1 monoclonal antibody, clone 4D5 (Cat # MAB10619) against CTNNB1-hIgGFc transfected HEK293 cell lysate.
Immunohistochemical analysis of paraffin-embedded human placenta tissues using CTNNB1 monoclonal antibody, clone 4D5 (Cat # MAB10619) .
Immunofluorescence
Immunofluorescence analysis of A-549 (upper) and SK-BR-3 (bottom) cells using CTNNB1 monoclonal antibody, clone 4D5 (Cat # MAB10619) (green) . Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.
ELISA
Flow Cytometry
Flow cytometric analysis of A-549 cells using CTNNB1 monoclonal antibody, clone 4D5 (Cat # MAB10619) (green) and negative control (purple) .
Beta-catenin is an adherens junction protein. Adherens junctions (AJs; also called the zonula adherens) are critical for the establishment and maintenance of epithelial layers, such as those lining organ surfaces. AJs mediate adhesion between cells, communicate a signal that neighboring cells are present, and anchor the actin cytoskeleton. In serving these roles, AJs regulate normal cell growth and behavior. At several stages of embryogenesis, wound healing, and tumor cell metastasis, cells form and leave epithelia. This process, which involves the disruption and reestablishment of epithelial cell-cell contacts, may be regulated by the disassembly and assembly of AJs. AJs may also function in the transmission of the 'contact inhibition' signal, which instructs cells to stop dividing once an epithelial sheet is complete.[supplied by OMIM