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Last updated: 2014/12/14
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CTNNB1 monoclonal antibody, clone 4D5

  • Catalog # : MAB10619
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against partial recombinant CTNNB1.
  • Immunogen:
  • Recombinant protein corresponding to human CTNNB1.
  • Host:
  • Mouse
  • Theoretical MW (kDa):
  • 85
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Isotype:
  • IgG1
  • Recommend Usage:
  • ELISA (1:10000)
    Western Blot (1:500-1:2000)
    Immunohistochemistry (1:200-1:1000)
    Immunofluorescence (1:200-1:1000)
    Flow cytometry (1:200-1:400)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In ascites (0.03% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • Western blot analysis using CTNNB1 monoclonal antibody, clone 4D5 (Cat # MAB10619) against CTNNB1-hIgGFc transfected HEK293 cell lysate.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical analysis of paraffin-embedded human placenta tissues using CTNNB1 monoclonal antibody, clone 4D5 (Cat # MAB10619) .
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescence analysis of A-549 (upper) and SK-BR-3 (bottom) cells using CTNNB1 monoclonal antibody, clone 4D5 (Cat # MAB10619) (green) . Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.
  • ELISA
  • Flow Cytometry
  • Flow Cytometry
  • Flow cytometric analysis of A-549 cells using CTNNB1 monoclonal antibody, clone 4D5 (Cat # MAB10619) (green) and negative control (purple) .
  • Application Image
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 1499
  • Gene Name:
  • CTNNB1
  • Gene Alias:
  • CTNNB,DKFZp686D02253,FLJ25606,FLJ37923
  • Gene Description:
  • catenin (cadherin-associated protein), beta 1, 88kDa
  • Gene Summary:
  • Beta-catenin is an adherens junction protein. Adherens junctions (AJs; also called the zonula adherens) are critical for the establishment and maintenance of epithelial layers, such as those lining organ surfaces. AJs mediate adhesion between cells, communicate a signal that neighboring cells are present, and anchor the actin cytoskeleton. In serving these roles, AJs regulate normal cell growth and behavior. At several stages of embryogenesis, wound healing, and tumor cell metastasis, cells form and leave epithelia. This process, which involves the disruption and reestablishment of epithelial cell-cell contacts, may be regulated by the disassembly and assembly of AJs. AJs may also function in the transmission of the 'contact inhibition' signal, which instructs cells to stop dividing once an epithelial sheet is complete.[supplied by OMIM
  • Other Designations:
  • OTTHUMP00000165222,OTTHUMP00000165223,catenin (cadherin-associated protein), beta 1 (88kD),catenin beta-1
  • Interactome
  • Interactome
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