CRTC2 monoclonal antibody, clone 5B10
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More Files
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant CRTC2.
Immunogen
Recombinant protein corresponding to human CRTC2.
Host
Mouse
Theoretical MW (kDa)
80
Reactivity
Human, Monkey
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry (1:200-1:1000)
Immunofluorescence (1:200-1:1000)
Flow cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In ascites (0.03% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis using CRTC2 monoclonal antibody, clone 5B10 (Cat # MAB10615) against HeLa (1) and HEK293 (2) cell lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human ovary tumour tissues (upper) and lung cancer (bottom) using CRTC2 monoclonal antibody, clone 5B10 (Cat # MAB10615) with DAB staining.Immunofluorescence
Immunofluorescence analysis of HeLa cells using CRTC2 monoclonal antibody, clone 5B10 (Cat # MAB10615) (green) . Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Flow cytometric analysis of HeLa cells using CRTC2 monoclonal antibody, clone 5B10 (Cat # MAB10615) (green) and negative control (purple) . -
Gene Info — CRTC2
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Interactome
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Disease
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