Mouse monoclonal antibody raised against partial recombinant CRTC2.
Recombinant protein corresponding to human CRTC2.
Theoretical MW (kDa):
ELISA (1:10000) Western Blot (1:500-1:2000) Immunohistochemistry (1:200-1:1000) Immunofluorescence (1:200-1:1000) Flow cytometry (1:200-1:400) The optimal working dilution should be determined by the end user.
In ascites (0.03% sodium azide)
Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Western Blot (Cell lysate)
Western blot analysis using CRTC2 monoclonal antibody, clone 5B10 (Cat # MAB10615) against HeLa (1) and HEK293 (2) cell lysate.
Immunohistochemical analysis of paraffin-embedded human ovary tumour tissues (upper) and lung cancer (bottom) using CRTC2 monoclonal antibody, clone 5B10 (Cat # MAB10615) with DAB staining.
Immunofluorescence analysis of HeLa cells using CRTC2 monoclonal antibody, clone 5B10 (Cat # MAB10615) (green) . Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Flow cytometric analysis of HeLa cells using CRTC2 monoclonal antibody, clone 5B10 (Cat # MAB10615) (green) and negative control (purple) .