KRT8 monoclonal antibody, clone 8A5D12
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis using KRT8 monoclonal antibody, clone 8A5D12 (Cat # MAB10601) against A-549 (1) , HeLa (2) , MCF-7 (3) , A-431 (4) and HepG2 (5) cell lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human kidney tissues using KRT8 monoclonal antibody, clone 8A5D12 (Cat # MAB10601) .
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma (A) , lung cancer (B) and ovarian cancer tissue (C) , showing membrane and cytoplasmic localization with DAB staining using KRT8 monoclonal antibody, clone 8A5D12 (Cat # MAB10601) .
Immunofluorescence
Confocal immunofluorescence analysis of methanol-fixed ECA109 cells (upper) and HepG2 cells (bottom) using KRT8 monoclonal antibody, clone 8A5D12 (Cat # MAB10601) (green) , showing cytoplasmic localization. Blue: DRAQ5 fluorescent DNA dye.
-
Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant KRT8.
Immunogen
Recombinant protein corresponding to amino acids 391-483 of human KRT8.
Host
Mouse
Theoretical MW (kDa)
54
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry (1:200-1:1000)
Immunofluorescence (1:200-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In ascites (0.03% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
Applications
Western Blot (Cell lysate)
Western blot analysis using KRT8 monoclonal antibody, clone 8A5D12 (Cat # MAB10601) against A-549 (1) , HeLa (2) , MCF-7 (3) , A-431 (4) and HepG2 (5) cell lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human kidney tissues using KRT8 monoclonal antibody, clone 8A5D12 (Cat # MAB10601) .Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma (A) , lung cancer (B) and ovarian cancer tissue (C) , showing membrane and cytoplasmic localization with DAB staining using KRT8 monoclonal antibody, clone 8A5D12 (Cat # MAB10601) .Immunofluorescence
Confocal immunofluorescence analysis of methanol-fixed ECA109 cells (upper) and HepG2 cells (bottom) using KRT8 monoclonal antibody, clone 8A5D12 (Cat # MAB10601) (green) , showing cytoplasmic localization. Blue: DRAQ5 fluorescent DNA dye.Enzyme-linked Immunoabsorbent Assay
-
Gene Info — KRT8
Entrez GeneID
3856Gene Name
KRT8
Gene Alias
CARD2, CK8, CYK8, K2C8, K8, KO
Gene Description
keratin 8
Gene Ontology
HyperlinkGene Summary
This gene is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis. [provided by RefSeq
Other Designations
cytokeratin 8|keratin, type II cytoskeletal 8
-
Interactome
-
Disease
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com