MKI67 monoclonal antibody, clone 8D5
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More Files
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Specification
Product Description
Mouse monoclonal antibody raised against synthetic peptide of MKI67.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to human MKI67.
Sequence
CEDLAGFKELFQTPG
Host
Mouse
Theoretical MW (kDa)
358
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry (1:200-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In ascites (0.03% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis using MKI67 monoclonal antibody, clone 8D5 (Cat # MAB10582) against HeLa (1) , MCF-7 (2) and Raji (3) cell lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human lung cancer (upper) and rectal cancer (bottom) using MKI67 monoclonal antibody, clone 8D5 (Cat # MAB10582) with DAB staining.Enzyme-linked Immunoabsorbent Assay
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Gene Info — MKI67
Entrez GeneID
4288Gene Name
MKI67
Gene Alias
KIA, Ki-67
Gene Description
antigen identified by monoclonal antibody Ki-67
Omim ID
176741Gene Ontology
HyperlinkGene Summary
This gene encodes a nuclear protein that is associated with and may be necessary for cellular proliferation. Alternatively spliced transcript variants have been described. A related pseudogene exists on chromosome X. [provided by RefSeq
Other Designations
OTTHUMP00000020739|proliferation-related Ki-67 antigen
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Interactome
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Disease
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