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Last updated: 2014/8/18
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CRK monoclonal antibody, clone 3G11E8

  • Catalog # : MAB10573
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against partial recombinant CRK.
  • Immunogen:
  • Recombinant protein corresponding to human CRK.
  • Host:
  • Mouse
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Isotype:
  • IgG2b
  • Recommend Usage:
  • ELISA (1:10000)
    Western Blot (1:500-1:2000)
    Immunohistochemistry (1:200-1:1000)
    Immunofluorescence (1:200-1:1000)
    Flow cytometry (1:200-1:400)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In ascites (0.03% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • Western blot analysis using CRK monoclonal antibody, clone 3G11E8 (Cat # MAB10573) against HEK293 (1) and CRK-hIgGFc transfected HEK293 (2) cell lysate.
  • Western Blot (Recombinant protein)
  • Western Blot (Recombinant protein)
  • Western blot analysis using CRK monoclonal antibody, clone 3G11E8 (Cat # MAB10573) against recombinant protein.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical analysis of paraffin-embedded human intima cancer (A) and human lung cancer (B) tissues using CRK monoclonal antibody, clone 3G11E8 (Cat # MAB10573) with DAB staining.
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescence analysis of 3T3-L1 cells using CRK monoclonal antibody, clone 3G11E8 (Cat # MAB10573) (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
  • ELISA
  • ELISA
  • ELISA detection with CRK monoclonal antibody, clone 3G11E8 (Cat # MAB10573).
  • Flow Cytometry
  • Flow Cytometry
  • Flow cytometric analysis of HeLa cells using CRK monoclonal antibody, clone 3G11E8 (Cat # MAB10573) (blue) and negative control (red).
  • Application Image
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • enlarge
  • Western Blot (Recombinant protein)
  • Western Blot (Recombinant protein)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 1398
  • Gene Name:
  • CRK
  • Gene Alias:
  • CRKII
  • Gene Description:
  • v-crk sarcoma virus CT10 oncogene homolog (avian)
  • Gene Summary:
  • This gene encodes a member of an adapter protein family that binds to several tyrosine-phosphorylated proteins. The product of this gene has several SH2 and SH3 domains (src-homology domains) and is involved in several signaling pathways, recruiting cytoplasmic proteins in the vicinity of tyrosine kinase through SH2-phosphotyrosine interaction. The N-terminal SH2 domain of this protein functions as a positive regulator of transformation whereas the C-terminal SH3 domain functions as a negative regulator of transformation. Two alternative transcripts encoding different isoforms with distinct biological activity have been described. [provided by RefSeq
  • Other Designations:
  • avian sarcoma virus CT10 (v-crk) oncogene homolog,v-crk avian sarcoma virus CT10 oncogene homolog,v-crk sarcoma virus CT10 oncogene homolog
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