CRK monoclonal antibody, clone 3G11C1
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More Files
- More Functions
Western Blot (Transfected lysate)
Western blot analysis using CRK monoclonal antibody, clone 3G11C1 (Cat # MAB10572) against HEK293 (1) and CRK-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot (Recombinant protein)
Western blot analysis using CRK monoclonal antibody, clone 3G11C1 (Cat # MAB10572) against recombinant protein.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human bladder cancer (A) and human rectum cancer (B) tissues using CRK monoclonal antibody, clone 3G11C1 (Cat # MAB10572) with DAB staining.
Immunofluorescence
Immunofluorescence analysis of 3T3-L1 cells using CRK monoclonal antibody, clone 3G11C1 (Cat # MAB10572) (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Enzyme-linked Immunoabsorbent Assay
ELISA detection with CRK monoclonal antibody, clone 3G11C1 (Cat # MAB10572).
Flow Cytometry
Flow cytometric analysis of MCF-7 cells using CRK monoclonal antibody, clone 3G11C1 (Cat # MAB10572) (blue) and negative control (red).
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant CRK.
Immunogen
Recombinant protein corresponding to human CRK.
Host
Mouse
Reactivity
Human
Form
Liquid
Isotype
IgG2b
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry (1:200-1:1000)
Immunofluorescence (1:200-1:1000)
Flow cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In ascites (0.03% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis using CRK monoclonal antibody, clone 3G11C1 (Cat # MAB10572) against HEK293 (1) and CRK-hIgGFc transfected HEK293 (2) cell lysate.Western Blot (Recombinant protein)
Western blot analysis using CRK monoclonal antibody, clone 3G11C1 (Cat # MAB10572) against recombinant protein.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human bladder cancer (A) and human rectum cancer (B) tissues using CRK monoclonal antibody, clone 3G11C1 (Cat # MAB10572) with DAB staining.Immunofluorescence
Immunofluorescence analysis of 3T3-L1 cells using CRK monoclonal antibody, clone 3G11C1 (Cat # MAB10572) (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.Enzyme-linked Immunoabsorbent Assay
ELISA detection with CRK monoclonal antibody, clone 3G11C1 (Cat # MAB10572).Flow Cytometry
Flow cytometric analysis of MCF-7 cells using CRK monoclonal antibody, clone 3G11C1 (Cat # MAB10572) (blue) and negative control (red). -
Gene Info — CRK
Entrez GeneID
1398Gene Name
CRK
Gene Alias
CRKII
Gene Description
v-crk sarcoma virus CT10 oncogene homolog (avian)
Omim ID
164762Gene Ontology
HyperlinkGene Summary
This gene encodes a member of an adapter protein family that binds to several tyrosine-phosphorylated proteins. The product of this gene has several SH2 and SH3 domains (src-homology domains) and is involved in several signaling pathways, recruiting cytoplasmic proteins in the vicinity of tyrosine kinase through SH2-phosphotyrosine interaction. The N-terminal SH2 domain of this protein functions as a positive regulator of transformation whereas the C-terminal SH3 domain functions as a negative regulator of transformation. Two alternative transcripts encoding different isoforms with distinct biological activity have been described. [provided by RefSeq
Other Designations
avian sarcoma virus CT10 (v-crk) oncogene homolog|v-crk avian sarcoma virus CT10 oncogene homolog|v-crk sarcoma virus CT10 oncogene homolog
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Interactome
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Pathway
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