CLOCK monoclonal antibody, clone 8F7
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More Files
- More Functions
Western Blot (Transfected lysate)
Western blot analysis using CLOCK monoclonal antibody, clone 8F7 (Cat # MAB10570) against CLOCK-hIgGFc transfected HEK293 cell lysate.
Immunofluorescence
Immunofluorescence analysis of HeLa cells using CLOCK monoclonal antibody, clone 8F7 (Cat # MAB10570) (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Blue: DRAQ5 fluorescent DNA dye.
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant CLOCK.
Immunogen
Recombinant protein corresponding to human CLOCK.
Host
Mouse
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunofluorescence (1:200-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In ascites (0.03% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis using CLOCK monoclonal antibody, clone 8F7 (Cat # MAB10570) against CLOCK-hIgGFc transfected HEK293 cell lysate.Immunofluorescence
Immunofluorescence analysis of HeLa cells using CLOCK monoclonal antibody, clone 8F7 (Cat # MAB10570) (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Blue: DRAQ5 fluorescent DNA dye.Enzyme-linked Immunoabsorbent Assay
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Gene Info — CLOCK
Entrez GeneID
9575Gene Name
CLOCK
Gene Alias
KAT13D, KIAA0334, bHLHe8
Gene Description
clock homolog (mouse)
Omim ID
601851Gene Ontology
HyperlinkGene Summary
This gene encodes a protein that belongs to the basic helix-loop-helix (bHLH) family of transcription factors. Polymorphisms within the encoded protein have been associated with circadian rhythm sleep disorders. A similar protein in mice is a circadian regulator that acts as a transcription factor and forms a heterodimer with aryl hydrocarbon receptor nuclear translocator-like to activate transcription of mouse period 1. [provided by RefSeq
Other Designations
OTTHUMP00000128334|circadian locomoter output cycles kaput protein|clock
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Interactome
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Pathway
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Disease
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