Mouse monoclonal antibody raised against partial recombinant CLOCK.
Recombinant protein corresponding to human CLOCK.
ELISA (1:10000) Western Blot (1:500-1:2000) Immunofluorescence (1:200-1:1000) The optimal working dilution should be determined by the end user.
In ascites (0.03% sodium azide)
Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Western Blot (Transfected lysate)
Western blot analysis using CLOCK monoclonal antibody, clone 8F7 (Cat # MAB10570) against CLOCK-hIgGFc transfected HEK293 cell lysate.
Immunofluorescence analysis of HeLa cells using CLOCK monoclonal antibody, clone 8F7 (Cat # MAB10570) (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Blue: DRAQ5 fluorescent DNA dye.
This gene encodes a protein that belongs to the basic helix-loop-helix (bHLH) family of transcription factors. Polymorphisms within the encoded protein have been associated with circadian rhythm sleep disorders. A similar protein in mice is a circadian regulator that acts as a transcription factor and forms a heterodimer with aryl hydrocarbon receptor nuclear translocator-like to activate transcription of mouse period 1. [provided by RefSeq