CDC2 monoclonal antibody, clone 8C5A7F10
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis using CDC2 monoclonal antibody, clone 8C5A7F10 (Cat # MAB10544) against Jurkat cell lysate.
Immunofluorescence
Immunofluorescence analysis of HeLa cells using CDC2 monoclonal antibody, clone 8C5A7F10 (Cat # MAB10544) (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Flow Cytometry
Flow cytometric analysis of PC-2 cells using CDC2 monoclonal antibody, clone 8C5A7F10 (Cat # MAB10544) (green) and negative control (purple).
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant CDC2.
Immunogen
Recombinant protein corresponding to human CDC2.
Host
Mouse
Theoretical MW (kDa)
34
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunofluorescence (1:200-1:1000)
Flow cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In ascites (0.03% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis using CDC2 monoclonal antibody, clone 8C5A7F10 (Cat # MAB10544) against Jurkat cell lysate.Immunofluorescence
Immunofluorescence analysis of HeLa cells using CDC2 monoclonal antibody, clone 8C5A7F10 (Cat # MAB10544) (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Flow cytometric analysis of PC-2 cells using CDC2 monoclonal antibody, clone 8C5A7F10 (Cat # MAB10544) (green) and negative control (purple). -
Gene Info — CDC2
Entrez GeneID
983Gene Name
CDC2
Gene Alias
CDC28A, CDK1, DKFZp686L20222, MGC111195
Gene Description
cell division cycle 2, G1 to S and G2 to M
Omim ID
116940Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with this protein and function as regulatory subunits. The kinase activity of this protein is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of this protein also play important regulatory roles in cell cycle control. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
OTTHUMP00000019660|cell cycle controller CDC2|cell division control protein 2 homolog|cell division cycle 2 protein|cyclin-dependent kinase 1|p34 protein kinase
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Interactome
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Pathway
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Disease
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