Mouse monoclonal antibody raised against partial recombinant CD44.
Recombinant protein corresponding to amino acids 628-699 of human CD44.
Theoretical MW (kDa):
ELISA (1:10000) Western Blot (1:500-1:2000) Immunohistochemistry (1:200-1:1000) Immunofluorescence (1:200-1:1000) Flow cytometry (1:200-1:400) The optimal working dilution should be determined by the end user.
In ascites (0.03% sodium azide)
Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissues, showing membrane localization with DAB staining using CD44 monoclonal antibody, clone 8E2F3 (Cat # MAB10538).
Confocal analysis of paraffin-embedded human lung cancer tissues using CD44 monoclonal antibody, clone 8E2F3 (Cat # MAB10538) (green), showing membrane localization. Blue: DRAQ5 fluorescent DNA dye.
Confocal immunofluorescence analysis of PANC-1 cells using CD44 monoclonal antibody, clone 8E2F3 (Cat # MAB10538) (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.
Confocal immunofluorescence analysis of methanol-fixed A-431 (A), HeLa (B), PANC-1 (C) and EC (D) cells using CD44 monoclonal antibody, clone 8E2F3 (Cat # MAB10538) (green), showing membrane localization. Blue: DRAQ5 fluorescent DNA dye.
Flow cytometric analysis of HeLa cells using CD44 monoclonal antibody, clone 8E2F3 (Cat # MAB10538) (A) and negative control (B).
The protein encoded by this gene is a cell-surface glycoprotein involved in cell-cell interactions, cell adhesion and migration. It is a receptor for hyaluronic acid (HA) and can also interact with other ligands, such as osteopontin, collagens, and matrix metalloproteinases (MMPs). This protein participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing, hematopoiesis, and tumor metastasis. Transcripts for this gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full length nature of some of these variants has not been determined. Alternative splicing is the basis for the structural and functional diversity of this protein, and may be related to tumor metastasis. [provided by RefSeq
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