Product Browser

Last updated: 2014/9/15
  • Related Product Showcase
  • By Application

Product Compare

Product Compare Cancel Click this icon to add products to compare list. Select up to 10 products.

Quick Order (Tutorial)

Input Catalog #,
place order here!
Catalog # :
  • Where to buy
  • Choose your location

CD44 monoclonal antibody, clone 8E2F3

  • Catalog # : MAB10538
  • Visit Frequency :
  • Countries :
  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against partial recombinant CD44.
  • Immunogen:
  • Recombinant protein corresponding to amino acids 628-699 of human CD44.
  • Host:
  • Mouse
  • Theoretical MW (kDa):
  • 82
  • Reactivity:
  • Human, Mouse
  • Form:
  • Liquid
  • Isotype:
  • IgG1
  • Recommend Usage:
  • ELISA (1:10000)
    Western Blot (1:500-1:2000)
    Immunohistochemistry (1:200-1:1000)
    Immunofluorescence (1:200-1:1000)
    Flow cytometry (1:200-1:400)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In ascites (0.03% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis using CD44 monoclonal antibody, clone 8E2F3 (Cat # MAB10538) against HeLa (1) and HUVE-12(2) cell lysate.
  • Western Blot (Recombinant protein)
  • Western Blot (Recombinant protein)
  • Western blot analysis using CD44 monoclonal antibody, clone 8E2F3 (Cat # MAB10538) against truncated Trx-CD44 recombinant protein (1) and GST-CD44 (aa 628-699) recombinant protein (2).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissues, showing membrane localization with DAB staining using CD44 monoclonal antibody, clone 8E2F3 (Cat # MAB10538).
  • Immunofluorescence
  • Immunofluorescence
  • Confocal analysis of paraffin-embedded human lung cancer tissues using CD44 monoclonal antibody, clone 8E2F3 (Cat # MAB10538) (green), showing membrane localization. Blue: DRAQ5 fluorescent DNA dye.
  • Immunofluorescence
  • Immunofluorescence
  • Confocal immunofluorescence analysis of PANC-1 cells using CD44 monoclonal antibody, clone 8E2F3 (Cat # MAB10538) (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.
  • Immunofluorescence
  • Immunofluorescence
  • Confocal immunofluorescence analysis of methanol-fixed A-431 (A), HeLa (B), PANC-1 (C) and EC (D) cells using CD44 monoclonal antibody, clone 8E2F3 (Cat # MAB10538) (green), showing membrane localization. Blue: DRAQ5 fluorescent DNA dye.
  • ELISA
  • Flow Cytometry
  • Flow Cytometry
  • Flow cytometric analysis of HeLa cells using CD44 monoclonal antibody, clone 8E2F3 (Cat # MAB10538) (A) and negative control (B).
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Western Blot (Recombinant protein)
  • Western Blot (Recombinant protein)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 960
  • Gene Name:
  • CD44
  • Gene Alias:
  • CDW44,CSPG8,ECMR-III,HCELL,IN,LHR,MC56,MDU2,MDU3,MGC10468,MIC4,MUTCH-I,Pgp1
  • Gene Description:
  • CD44 molecule (Indian blood group)
  • Gene Summary:
  • The protein encoded by this gene is a cell-surface glycoprotein involved in cell-cell interactions, cell adhesion and migration. It is a receptor for hyaluronic acid (HA) and can also interact with other ligands, such as osteopontin, collagens, and matrix metalloproteinases (MMPs). This protein participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing, hematopoiesis, and tumor metastasis. Transcripts for this gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full length nature of some of these variants has not been determined. Alternative splicing is the basis for the structural and functional diversity of this protein, and may be related to tumor metastasis. [provided by RefSeq
  • Other Designations:
  • CD44 antigen,CD44 antigen (homing function and Indian blood group system),CDW44 antigen,GP90 lymphocyte homing/adhesion receptor,Hermes antigen,antigen gp90 homing receptor,cell adhesion molecule,cell surface glycoprotein CD44,chondroitin sulfate proteogl
  • RSS
  • YouTube
  • Linkedin
  • Facebook