CD3E monoclonal antibody, clone 4E2
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis using CD3E monoclonal antibody, clone 4E2 (Cat # MAB10536) against Jurkat cell lysate.
Flow Cytometry
Flow cytometric analysis of Jurkat cells using CD3E monoclonal antibody, clone 4E2 (Cat # MAB10536) (green) and negative control (purple).
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant CD3E.
Immunogen
Recombinant protein corresponding to human CD3E.
Host
Mouse
Theoretical MW (kDa)
23
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Flow cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In ascites (0.03% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis using CD3E monoclonal antibody, clone 4E2 (Cat # MAB10536) against Jurkat cell lysate.Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Flow cytometric analysis of Jurkat cells using CD3E monoclonal antibody, clone 4E2 (Cat # MAB10536) (green) and negative control (purple). -
Gene Info — CD3E
Entrez GeneID
916Gene Name
CD3E
Gene Alias
FLJ18683, T3E, TCRE
Gene Description
CD3e molecule, epsilon (CD3-TCR complex)
Omim ID
186830Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is the CD3-epsilon polypeptide, which together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. Defects in this gene cause immunodeficiency. This gene has also been linked to a susceptibility to type I diabetes in women. [provided by RefSeq
Other Designations
CD3-epsilon|CD3E antigen, epsilon polypeptide|CD3e antigen, epsilon polypeptide (TiT3 complex)|T-cell antigen receptor complex, epsilon subunit of T3|T-cell surface antigen T3/Leu-4 epsilon chain|T-cell surface glycoprotein CD3 epsilon chain
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Interactome
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Pathway
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Disease
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