Mouse monoclonal antibody raised against partial recombinant CBL.
Recombinant protein corresponding to human CBL.
Theoretical MW (kDa):
Human, Mouse, Rat
ELISA (1:10000) Western Blot (1:500-1:2000) Immunohistochemistry (1:200-1:1000) Immunofluorescence (1:200-1:1000) Flow cytometry (1:200-1:400) The optimal working dilution should be determined by the end user.
In ascites (0.03% sodium azide)
Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Western Blot (Cell lysate)
Western blot analysis using CBL monoclonal antibody, clone 3B12 (Cat # MAB10513) against Raji (1), Raw 264.7 (2), K-562 (3), SK-BR-3 (4), 3T3-L1 (5), THP-1 (6) and PC-12 (7) cell lysate.
Western Blot (Recombinant protein)
Western blot analysis using CBL monoclonal antibody, clone 3B12 (Cat # MAB10513) against recombinant protein.
Immunohistochemical analysis of paraffin-embedded human ovarian cancer (A) and human bladder cancer (B) tissues using CBL monoclonal antibody, clone 3B12 (Cat # MAB10513) with DAB staining.
Immunofluorescence analysis of HeLa cells using CBL monoclonal antibody, clone 3B12 (Cat # MAB10513) (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
The cbl oncogene was first identified as part of a transforming retrovirus which induces mouse pre-B and pro-B cell lymphomas. As an adaptor protein for receptor protein-tyrosine kinases, it positively regulates receptor protein-tyrosine kinase ubiquitination in a manner dependent upon its variant SH2 and RING finger domains. Ubiquitination of receptor protein-tyrosine kinases terminates signaling by marking active receptors for degradation. [provided by RefSeq