CBL monoclonal antibody, clone 3B12
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis using CBL monoclonal antibody, clone 3B12 (Cat # MAB10513) against Raji (1), Raw 264.7 (2), K-562 (3), SK-BR-3 (4), 3T3-L1 (5), THP-1 (6) and PC-12 (7) cell lysate.
Western Blot (Recombinant protein)
Western blot analysis using CBL monoclonal antibody, clone 3B12 (Cat # MAB10513) against recombinant protein.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human ovarian cancer (A) and human bladder cancer (B) tissues using CBL monoclonal antibody, clone 3B12 (Cat # MAB10513) with DAB staining.
Immunofluorescence
Immunofluorescence analysis of HeLa cells using CBL monoclonal antibody, clone 3B12 (Cat # MAB10513) (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Enzyme-linked Immunoabsorbent Assay
ELISA detection with CBL monoclonal antibody, clone 3B12 (Cat # MAB10513).
Flow Cytometry
Flow cytometric analysis of MCF-7 cells using CBL monoclonal antibody, clone 3B12 (Cat # MAB10513) (blue) and negative control (red).
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant CBL.
Immunogen
Recombinant protein corresponding to human CBL.
Host
Mouse
Theoretical MW (kDa)
120
Reactivity
Human, Mouse, Rat
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry (1:200-1:1000)
Immunofluorescence (1:200-1:1000)
Flow cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In ascites (0.03% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis using CBL monoclonal antibody, clone 3B12 (Cat # MAB10513) against Raji (1), Raw 264.7 (2), K-562 (3), SK-BR-3 (4), 3T3-L1 (5), THP-1 (6) and PC-12 (7) cell lysate.Western Blot (Recombinant protein)
Western blot analysis using CBL monoclonal antibody, clone 3B12 (Cat # MAB10513) against recombinant protein.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human ovarian cancer (A) and human bladder cancer (B) tissues using CBL monoclonal antibody, clone 3B12 (Cat # MAB10513) with DAB staining.Immunofluorescence
Immunofluorescence analysis of HeLa cells using CBL monoclonal antibody, clone 3B12 (Cat # MAB10513) (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.Enzyme-linked Immunoabsorbent Assay
ELISA detection with CBL monoclonal antibody, clone 3B12 (Cat # MAB10513).Flow Cytometry
Flow cytometric analysis of MCF-7 cells using CBL monoclonal antibody, clone 3B12 (Cat # MAB10513) (blue) and negative control (red). -
Gene Info — CBL
Entrez GeneID
867Gene Name
CBL
Gene Alias
C-CBL, CBL2, RNF55
Gene Description
Cas-Br-M (murine) ecotropic retroviral transforming sequence
Omim ID
165360Gene Ontology
HyperlinkGene Summary
The cbl oncogene was first identified as part of a transforming retrovirus which induces mouse pre-B and pro-B cell lymphomas. As an adaptor protein for receptor protein-tyrosine kinases, it positively regulates receptor protein-tyrosine kinase ubiquitination in a manner dependent upon its variant SH2 and RING finger domains. Ubiquitination of receptor protein-tyrosine kinases terminates signaling by marking active receptors for degradation. [provided by RefSeq
Other Designations
oncogene CBL2
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Interactome
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Pathway
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Disease
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