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Last updated: 2014/12/14
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CBL monoclonal antibody, clone 3B12

  • Catalog # : MAB10513
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against partial recombinant CBL.
  • Immunogen:
  • Recombinant protein corresponding to human CBL.
  • Host:
  • Mouse
  • Theoretical MW (kDa):
  • 120
  • Reactivity:
  • Human, Mouse, Rat
  • Form:
  • Liquid
  • Isotype:
  • IgG1
  • Recommend Usage:
  • ELISA (1:10000)
    Western Blot (1:500-1:2000)
    Immunohistochemistry (1:200-1:1000)
    Immunofluorescence (1:200-1:1000)
    Flow cytometry (1:200-1:400)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In ascites (0.03% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis using CBL monoclonal antibody, clone 3B12 (Cat # MAB10513) against Raji (1), Raw 264.7 (2), K-562 (3), SK-BR-3 (4), 3T3-L1 (5), THP-1 (6) and PC-12 (7) cell lysate.
  • Western Blot (Recombinant protein)
  • Western Blot (Recombinant protein)
  • Western blot analysis using CBL monoclonal antibody, clone 3B12 (Cat # MAB10513) against recombinant protein.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical analysis of paraffin-embedded human ovarian cancer (A) and human bladder cancer (B) tissues using CBL monoclonal antibody, clone 3B12 (Cat # MAB10513) with DAB staining.
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescence analysis of HeLa cells using CBL monoclonal antibody, clone 3B12 (Cat # MAB10513) (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
  • ELISA
  • ELISA
  • ELISA detection with CBL monoclonal antibody, clone 3B12 (Cat # MAB10513).
  • Flow Cytometry
  • Flow Cytometry
  • Flow cytometric analysis of MCF-7 cells using CBL monoclonal antibody, clone 3B12 (Cat # MAB10513) (blue) and negative control (red).
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Western Blot (Recombinant protein)
  • Western Blot (Recombinant protein)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 867
  • Gene Name:
  • CBL
  • Gene Alias:
  • C-CBL,CBL2,RNF55
  • Gene Description:
  • Cas-Br-M (murine) ecotropic retroviral transforming sequence
  • Gene Summary:
  • The cbl oncogene was first identified as part of a transforming retrovirus which induces mouse pre-B and pro-B cell lymphomas. As an adaptor protein for receptor protein-tyrosine kinases, it positively regulates receptor protein-tyrosine kinase ubiquitination in a manner dependent upon its variant SH2 and RING finger domains. Ubiquitination of receptor protein-tyrosine kinases terminates signaling by marking active receptors for degradation. [provided by RefSeq
  • Other Designations:
  • oncogene CBL2
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