ACTB monoclonal antibody, clone 8H10D10
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis using ACTB monoclonal antibody, clone 8H10D10 (Cat # MAB10501) against NIH/3T3 (1), Jurkat (2), HeLa (3), CHO (4), PC-12 (5), HEK293 (6), COS (7), A-549 (8) and MCF-7 (9) cell lysate.
Immunofluorescence
Confocal immunofluorescence analysis of SK-BR-3 (upper) and A-549 (bottom) cells using ACTB monoclonal antibody, clone 8H10D10 (Cat # MAB10501) (red, the secondary Ab is Cy3-Goat anti mouse IgG). Blue: DRAQ5 fluorescent DNA dye.
Flow Cytometry
Flow cytometric analysis of MCF-7 cells using ACTB monoclonal antibody, clone 8H10D10 (Cat # MAB10501) (A) and negative control (B).
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Specification
Product Description
Mouse monoclonal antibody raised against synthetic peptide of ACTB.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to human ACTB.
Host
Mouse
Theoretical MW (kDa)
42
Reactivity
Hamster, Human, Monkey, Mouse, Rat
Form
Liquid
Isotype
IgG2b
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunofluorescence (1:200-1:1000)
Flow cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In ascites (0.03% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis using ACTB monoclonal antibody, clone 8H10D10 (Cat # MAB10501) against NIH/3T3 (1), Jurkat (2), HeLa (3), CHO (4), PC-12 (5), HEK293 (6), COS (7), A-549 (8) and MCF-7 (9) cell lysate.Immunofluorescence
Confocal immunofluorescence analysis of SK-BR-3 (upper) and A-549 (bottom) cells using ACTB monoclonal antibody, clone 8H10D10 (Cat # MAB10501) (red, the secondary Ab is Cy3-Goat anti mouse IgG). Blue: DRAQ5 fluorescent DNA dye.Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Flow cytometric analysis of MCF-7 cells using ACTB monoclonal antibody, clone 8H10D10 (Cat # MAB10501) (A) and negative control (B). -
Gene Info — ACTB
Entrez GeneID
60Gene Name
ACTB
Gene Alias
PS1TP5BP1
Gene Description
actin, beta
Omim ID
102630Gene Ontology
HyperlinkGene Summary
This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. [provided by RefSeq
Other Designations
PS1TP5-binding protein 1|actin, cytoplasmic 1|beta actin|beta cytoskeletal actin
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Interactome
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Pathway
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Disease
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