BECN1 monoclonal antibody, clone 2A4
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis using BECN1 monoclonal antibody, clone 2A4 (Cat # MAB10500) against HeLa (1), A-431 (2), MCF-7 (3), Raji (4), Jurkat (5) and SK-BR-3 (6) cell lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human breast cancer tissues (A) and liver cancer tissues (B) using BECN1 monoclonal antibody, clone 2A4 (Cat # MAB10500) with DAB staining.
Enzyme-linked Immunoabsorbent Assay
ELISA detection with BECN1 monoclonal antibody, clone 2A4 (Cat # MAB10500).
Flow Cytometry
Flow cytometric analysis of Raji cells using BECN1 monoclonal antibody, clone 2A4 (Cat # MAB10500) (green) and negative control (purple).
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant BECN1.
Immunogen
Recombinant protein corresponding to human BECN1.
Host
Mouse
Theoretical MW (kDa)
60
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry (1:200-1:1000)
Flow cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In ascites (0.03% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis using BECN1 monoclonal antibody, clone 2A4 (Cat # MAB10500) against HeLa (1), A-431 (2), MCF-7 (3), Raji (4), Jurkat (5) and SK-BR-3 (6) cell lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human breast cancer tissues (A) and liver cancer tissues (B) using BECN1 monoclonal antibody, clone 2A4 (Cat # MAB10500) with DAB staining.Enzyme-linked Immunoabsorbent Assay
ELISA detection with BECN1 monoclonal antibody, clone 2A4 (Cat # MAB10500).Flow Cytometry
Flow cytometric analysis of Raji cells using BECN1 monoclonal antibody, clone 2A4 (Cat # MAB10500) (green) and negative control (purple). -
Gene Info — BECN1
Entrez GeneID
8678Gene Name
BECN1
Gene Alias
ATG6, VPS30, beclin1
Gene Description
beclin 1, autophagy related
Omim ID
604378Gene Ontology
HyperlinkGene Summary
O
Other Designations
ATG6 autophagy related 6 homolog|beclin 1|beclin 1 (coiled-coil, moesin-like BCL2 interacting protein)|beclin 1 (coiled-coil, moesin-like BCL2-interacting protein)
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Interactome
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Pathway
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Disease
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