BCL2 monoclonal antibody, clone 8E12
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More Files
- More Functions
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human colon cancer tissues (A) and human brain tissues (B) using BCL2 monoclonal antibody, clone 8E12 (Cat # MAB10499) with DAB staining.
Immunofluorescence
Immunofluorescence analysis of NIH/3T3 cells using BCL2 monoclonal antibody, clone 8E12 (Cat # MAB10499) (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Flow Cytometry
Flow cytometric analysis of 3T3-L1 cells using BCL2 monoclonal antibody, clone 8E12 (Cat # MAB10499) (green) and negative control (purple).
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Specification
Product Description
Mouse monoclonal antibody raised against synthetic peptide of BCL2.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to human BCL2.
Host
Mouse
Theoretical MW (kDa)
26
Reactivity
Human, Mouse
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Immunohistochemistry (1:200-1:1000)
Immunofluorescence (1:200-1:1000)
Flow cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In ascites (0.03% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human colon cancer tissues (A) and human brain tissues (B) using BCL2 monoclonal antibody, clone 8E12 (Cat # MAB10499) with DAB staining.Immunofluorescence
Immunofluorescence analysis of NIH/3T3 cells using BCL2 monoclonal antibody, clone 8E12 (Cat # MAB10499) (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Flow cytometric analysis of 3T3-L1 cells using BCL2 monoclonal antibody, clone 8E12 (Cat # MAB10499) (green) and negative control (purple). -
Gene Info — BCL2
Entrez GeneID
596Gene Name
BCL2
Gene Alias
Bcl-2
Gene Description
B-cell CLL/lymphoma 2
Omim ID
151430Gene Ontology
HyperlinkGene Summary
This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Two transcript variants, produced by alternate splicing, differ in their C-terminal ends. [provided by RefSeq
Other Designations
B-cell lymphoma protein 2|OTTHUMP00000163680
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Interactome
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Pathway
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Disease
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