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Last updated: 2014/7/28
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AURKA monoclonal antibody, clone 1F8

  • Catalog # : MAB10497
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against partial recombinant AURKA.
  • Immunogen:
  • Recombinant protein corresponding to human AURKA.
  • Host:
  • Mouse
  • Theoretical MW (kDa):
  • 48
  • Reactivity:
  • Human, Monkey, Rat
  • Form:
  • Liquid
  • Isotype:
  • IgG1
  • Recommend Usage:
  • ELISA (1:10000)
    Western Blot (1:500-1:2000)
    Immunofluorescence (1:200-1:1000)
    Flow cytometry (1:200-1:400)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In ascites (0.03% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis using AURKA monoclonal antibody, clone 1F8 (Cat # MAB10497) against HEK293 (1), SW-620 (2), MCF-7 (3), Jurkat (4), HeLa (5), HepG2 (6), COS-7 (7) and PC-12 (8) cell lysate.
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescence analysis of HeLa cells using AURKA monoclonal antibody, clone 1F8 (Cat # MAB10497) (green). Blue: DRAQ5 fluorescent DNA dye.
  • ELISA
  • ELISA
  • ELISA detection with AURKA monoclonal antibody, clone 1F8 (Cat # MAB10497).
  • Flow Cytometry
  • Flow Cytometry
  • Flow cytometric analysis of K-562 cells using AURKA monoclonal antibody, clone 1F8 (Cat # MAB10497) (green) and negative control (purple).
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 6790
  • Gene Name:
  • AURKA
  • Gene Alias:
  • AIK,ARK1,AURA,AURORA2,BTAK,MGC34538,STK15,STK6,STK7
  • Gene Description:
  • aurora kinase A
  • Gene Summary:
  • The protein encoded by this gene is a cell cycle-regulated kinase that appears to be involved in microtubule formation and/or stabilization at the spindle pole during chromosome segregation. The encoded protein is found at the centrosome in interphase cells and at the spindle poles in mitosis. This gene may play a role in tumor development and progression. A processed pseudogene of this gene has been found on chromosome 1, and an unprocessed pseudogene has been found on chromosome 10. Multiple transcript variants encoding the same protein have been found for this gene. [provided by RefSeq
  • Other Designations:
  • IPL1-related kinase,OTTHUMP00000031340,OTTHUMP00000031342,OTTHUMP00000031343,OTTHUMP00000031344,OTTHUMP00000031345,OTTHUMP00000166071,aurora-A,aurora-related kinase 1,aurora/IPL1-like kinase,breast-tumor-amplified kinase,serine/threonine kinase 15,serine/
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