AURKA monoclonal antibody, clone 1F8
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis using AURKA monoclonal antibody, clone 1F8 (Cat # MAB10497) against HEK293 (1), SW-620 (2), MCF-7 (3), Jurkat (4), HeLa (5), HepG2 (6), COS-7 (7) and PC-12 (8) cell lysate.
Immunofluorescence
Immunofluorescence analysis of HeLa cells using AURKA monoclonal antibody, clone 1F8 (Cat # MAB10497) (green). Blue: DRAQ5 fluorescent DNA dye.
Enzyme-linked Immunoabsorbent Assay
ELISA detection with AURKA monoclonal antibody, clone 1F8 (Cat # MAB10497).
Flow Cytometry
Flow cytometric analysis of K-562 cells using AURKA monoclonal antibody, clone 1F8 (Cat # MAB10497) (green) and negative control (purple).
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant AURKA.
Immunogen
Recombinant protein corresponding to human AURKA.
Host
Mouse
Theoretical MW (kDa)
48
Reactivity
Human, Monkey, Rat
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunofluorescence (1:200-1:1000)
Flow cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In ascites (0.03% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis using AURKA monoclonal antibody, clone 1F8 (Cat # MAB10497) against HEK293 (1), SW-620 (2), MCF-7 (3), Jurkat (4), HeLa (5), HepG2 (6), COS-7 (7) and PC-12 (8) cell lysate.Immunofluorescence
Immunofluorescence analysis of HeLa cells using AURKA monoclonal antibody, clone 1F8 (Cat # MAB10497) (green). Blue: DRAQ5 fluorescent DNA dye.Enzyme-linked Immunoabsorbent Assay
ELISA detection with AURKA monoclonal antibody, clone 1F8 (Cat # MAB10497).Flow Cytometry
Flow cytometric analysis of K-562 cells using AURKA monoclonal antibody, clone 1F8 (Cat # MAB10497) (green) and negative control (purple). -
Gene Info — AURKA
Entrez GeneID
6790Gene Name
AURKA
Gene Alias
AIK, ARK1, AURA, AURORA2, BTAK, MGC34538, STK15, STK6, STK7
Gene Description
aurora kinase A
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a cell cycle-regulated kinase that appears to be involved in microtubule formation and/or stabilization at the spindle pole during chromosome segregation. The encoded protein is found at the centrosome in interphase cells and at the spindle poles in mitosis. This gene may play a role in tumor development and progression. A processed pseudogene of this gene has been found on chromosome 1, and an unprocessed pseudogene has been found on chromosome 10. Multiple transcript variants encoding the same protein have been found for this gene. [provided by RefSeq
Other Designations
IPL1-related kinase|OTTHUMP00000031340|OTTHUMP00000031342|OTTHUMP00000031343|OTTHUMP00000031344|OTTHUMP00000031345|OTTHUMP00000166071|aurora-A|aurora-related kinase 1|aurora/IPL1-like kinase|breast-tumor-amplified kinase|serine/threonine kinase 15|serine/
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Interactome
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Disease
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