ACTA2 monoclonal antibody, clone 1H8
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis using ACTA2 monoclonal antibody, clone 1H8 (Cat # MAB10480) against HeLa (1), Jurkta (2), HepG2 (3), MCF-7 (4), A-431 (5), A-549 (6), PC-12 (7), NIH/3T3 (8) and COS-7 (9) cell lysate.
Immunofluorescence
Immunofluorescence analysis of NIH/3T3 cells using ACTA2 monoclonal antibody, clone 1H8 (Cat # MAB10480) (green). Blue: DRAQ5 fluorescent DNA dye.
Enzyme-linked Immunoabsorbent Assay
ELISA detection with ACTA2 monoclonal antibody, clone 1H8 (Cat # MAB10480).
Flow Cytometry
Flow cytometric analysis of HeLa cells using ACTA2 monoclonal antibody, clone 1H8 (Cat # MAB10480) (green) and negative control (purple).
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant ACTA2.
Immunogen
Recombinant protein corresponding to human ACTA2.
Host
Mouse
Theoretical MW (kDa)
42
Reactivity
Human, Monkey, Mouse, Rat
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunofluorescence (1:200-1:1000)
Flow cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In ascites (0.03% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis using ACTA2 monoclonal antibody, clone 1H8 (Cat # MAB10480) against HeLa (1), Jurkta (2), HepG2 (3), MCF-7 (4), A-431 (5), A-549 (6), PC-12 (7), NIH/3T3 (8) and COS-7 (9) cell lysate.Immunofluorescence
Immunofluorescence analysis of NIH/3T3 cells using ACTA2 monoclonal antibody, clone 1H8 (Cat # MAB10480) (green). Blue: DRAQ5 fluorescent DNA dye.Enzyme-linked Immunoabsorbent Assay
ELISA detection with ACTA2 monoclonal antibody, clone 1H8 (Cat # MAB10480).Flow Cytometry
Flow cytometric analysis of HeLa cells using ACTA2 monoclonal antibody, clone 1H8 (Cat # MAB10480) (green) and negative control (purple). -
Gene Info — ACTA2
Entrez GeneID
59Gene Name
ACTA2
Gene Alias
AAT6, ACTSA
Gene Description
actin, alpha 2, smooth muscle, aorta
Omim ID
102620Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Defects in this gene cause aortic aneurysm familial thoracic type 6. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq
Other Designations
OTTHUMP00000020042|alpha 2 actin|alpha-cardiac actin|growth-inhibiting gene 46
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Interactome
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Pathway
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Disease
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