Mouse monoclonal antibody raised against recombinant BTK.
Immunogen:
Recombinant protein corresponding to human BTK.
Host:
Mouse
Theoretical MW (kDa):
77
Reactivity:
Human
Form:
Liquid
Recommend Usage:
Western Blot (1:500-1:2000) Immunohistochemistry (1:200-1:1000) Immunofluorescence (1:200-1:1000) ELISA (1:10000) The optimal working dilution should be determined by the end user.
Storage Buffer:
In ascitic (0.03% sodium azide)
Storage Instruction:
Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing.
Note:
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Applications
Western Blot (Cell lysate)
Western blot analysis of BTK monoclonal antibody, clone 7F12H4 (Cat # MAB10456) against K-562 (1), MCF-7 (2), Jurkat (3) and HEK293 (4) cell lysate.
Immunohistochemical analysis of paraffin-embedded human lymph-node tissue (A) and human lymph follicle tissue (B), showing cytoplasmic and membrane localization using BTK monoclonal antibody, clone 7F12H4 (Cat # MAB10456) with DAB staining.
Immunofluorescence
Immunofluorescence analysis of Jurkat cells using BTK monoclonal antibody, clone 7F12H4 (Cat # MAB10456).
The protein encoded by this gene plays a crucial role in B-cell development. Mutations in this gene cause X-linked agammaglobulinemia type 1, which is an immunodeficiency characterized by the failure to produce mature B lymphocytes, and associated with a failure of Ig heavy chain rearrangement. [provided by RefSeq