Mouse monoclonal antibody raised against recombinant EIF4EBP1.
Immunogen:
Recombinant protein corresponding to human EIF4EBP1.
Host:
Mouse
Reactivity:
Human
Form:
Liquid
Recommend Usage:
Immunohistochemistry (1:200-1:1000) Immunofluorescence (1:200-1:1000) ELISA (1:10000) The optimal working dilution should be determined by the end user.
Storage Buffer:
In PBS (0.03% sodium azide)
Storage Instruction:
Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing.
Note:
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Applications
Western Blot (Recombinant protein)
Western blot analysis using EIF4EBP1 monoclonal antibody, clone 11G12C11 (Cat # MAB10437) against truncated EIF4EBP1 recombinant protein (1).
Immunohistochemical analysis of paraffin-embedded human pancreas carcinoma (A), esophagus carcinoma tissue (B) and ovary tumor tissue, showing cytoplasmic and membrane localization using EIF4EBP1 monoclonal antibody, clone 11G12C11 (Cat # MAB10437) with DAB staining.
This gene encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation. [provided by RefSeq
Other Designations:
eIF4E-binding protein 1,phosphorylated heat- and acid-stable protein regulated by insulin 1