BRAF monoclonal antibody, clone 1H12
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of BRAF monoclonal antibody, clone 1H12 (Cat # MAB10422) against HeLa (1), HL-60 (2), HepG2 (3) and NIH/3T3 (4) cell lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human testis tissue using BRAF monoclonal antibody, clone 1H12 (Cat # MAB10422).
Immunofluorescence
Confocal immunofluorescence analysis of MCF-7 (A) and HepG2 (B) cells using BRAF monoclonal antibody, clone 1H12 (Cat # MAB10422) (green).
Red: Actin filaments have been labeled with DY-554 phalloidin.
Blue: DRAQ5 fluorescent DNA dye.
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant BRAF.
Immunogen
Recombinant protein corresponding to human BRAF.
Host
Mouse
Theoretical MW (kDa)
87
Reactivity
Human, Mouse
Form
Liquid
Isotype
IgG1
Recommend Usage
Western Blot (1:500-1:2000)
Immunohistochemistry (1:200-1:1000)
Immunofluorescence (1:200-1:1000)
ELISA (1:10000)
The optimal working dilution should be determined by the end user.Storage Buffer
In ascitic (0.03% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of BRAF monoclonal antibody, clone 1H12 (Cat # MAB10422) against HeLa (1), HL-60 (2), HepG2 (3) and NIH/3T3 (4) cell lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human testis tissue using BRAF monoclonal antibody, clone 1H12 (Cat # MAB10422).Immunofluorescence
Confocal immunofluorescence analysis of MCF-7 (A) and HepG2 (B) cells using BRAF monoclonal antibody, clone 1H12 (Cat # MAB10422) (green).
Red: Actin filaments have been labeled with DY-554 phalloidin.
Blue: DRAQ5 fluorescent DNA dye.Enzyme-linked Immunoabsorbent Assay
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Gene Info — BRAF
Entrez GeneID
673Gene Name
BRAF
Gene Alias
B-RAF1, BRAF1, FLJ95109, MGC126806, MGC138284, RAFB1
Gene Description
v-raf murine sarcoma viral oncogene homolog B1
Gene Ontology
HyperlinkGene Summary
This gene encodes a protein belonging to the raf/mil family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene are associated with cardiofaciocutaneous syndrome, a disease characterized by heart defects, mental retardation and a distinctive facial appearance. Mutations in this gene have also been associated with various cancers, including non-Hodgkin lymphoma, colorectal cancer, malignant melanoma, thyroid carcinoma, non-small cell lung carcinoma, and adenocarcinoma of lung. A pseudogene, which is located on chromosome X, has been identified for this gene. [provided by RefSeq
Other Designations
94 kDa B-raf protein|B-Raf proto-oncogene serine/threonine-protein kinase (p94)|Murine sarcoma viral (v-raf) oncogene homolog B1
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Interactome
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Pathway
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Disease
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