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XRCC5 monoclonal antibody, clone 5C5   

  • Catalog # : MAB10399
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  • 100 uL
  • USD $ 619
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  • Email : sales@abnova.com
    Phone : +1-909-992-0619 (new)
    Fax : +1-909-992-3401
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against recombinant XRCC5.
  • Immunogen:
  • Recombinant protein corresponding to human XRCC5.
  • Host:
  • Mouse
  • Theoretical MW (kDa):
  • 86
  • Reactivity:
  • Human, Mouse
  • Form:
  • Liquid
  • Recommend Usage:
  • ELISA (1:10000)
    Western Blot (1:500-1:2000)
    Immunohistochemistry (1:200-1:1000)
    Immunofluorescence (1:200-1:1000)
    Flow cytometry (1:200-1:400)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In ascites (0.03% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis of XRCC5 monoclonal antobody, clone 5C5 (Cat # MAB10399) against HeLa (1), MCF-7 (2), A-549 (3) and NIH/3T3 (4) cell lysate.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue (A) and human colon cancer tissue (B) using XRCC5 monoclonal antobody, clone 5C5 (Cat # MAB10399) with DAB staining.
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescence analysis of HeLa cells using XRCC5 monoclonal antobody, clone 5C5 (Cat # MAB10399) (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
  • ELISA
  • Flow Cytometry
  • Flow Cytometry
  • Flow cytometric analysis of HeLa cells using XRCC5 monoclonal antobody, clone 5C5 (Cat # MAB10399) (green) and negative control (purple).
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 7520
  • Gene Name:
  • XRCC5
  • Gene Alias:
  • FLJ39089,KARP-1,KARP1,KU80,KUB2,Ku86,NFIV
  • Gene Description:
  • X-ray repair complementing defective repair in Chinese hamster cells 5 (double-strand-break rejoining)
  • Gene Summary:
  • The protein encoded by this gene is the 80-kilodalton subunit of the Ku heterodimer protein which is also known as ATP-dependant DNA helicase II or DNA repair protein XRCC5. Ku is the DNA-binding component of the DNA-dependent protein kinase, and it functions together with the DNA ligase IV-XRCC4 complex in the repair of DNA double-strand break by non-homologous end joining and the completion of V(D)J recombination events. This gene functionally complements Chinese hamster xrs-6, a mutant defective in DNA double-strand break repair and in ability to undergo V(D)J recombination. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity. [provided by RefSeq
  • Other Designations:
  • ATP-dependent DNA helicase II,DNA repair protein XRCC5,Ku autoantigen, 80kDa,Ku86 autoantigen related protein 1,X-ray repair complementing defective repair in Chinese hamster cells 5 (double-strand-break rejoining; Ku autoantigen, 80kD),X-ray repair, comp
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