Mouse monoclonal antibody raised against recombinant SRC.
Immunogen:
Recombinant protein corresponding to human SRC.
Host:
Mouse
Theoretical MW (kDa):
60
Reactivity:
Human
Form:
Liquid
Recommend Usage:
ELISA (1:10000) Western Blot (1:500-1:2000) Immunohistochemistry (1:200-1:1000) Flow cytometry (1:200-1:400) The optimal working dilution should be determined by the end user.
Storage Buffer:
In ascites (0.03% sodium azide)
Storage Instruction:
Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing.
Note:
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Applications
Western Blot (Cell lysate)
Western blot analysis of SRC monoclonal antobody, clone 1F11 (Cat # MAB10395) against MCF-7 (1), A-431 (2), HeLa (3), HEK293 (4), NIH/3T3 (5), PC-12 (6) and COS-7 (7) cell lysate.
Western Blot (Recombinant protein)
Western blot analysis using SRC monoclonal antobody, clone 1F11 (Cat # MAB10395) against human SRC (aa 1-189) recombinant protein. (Expected MW is 47.8 kDa).
Immunohistochemical analysis of paraffin-embedded human bladder cancer (A) and human esophageal (B) tissue using SRC monoclonal antobody, clone 1F11 (Cat # MAB10395) with DAB staining.
ELISA
Flow Cytometry
Flow cytometric analysis of MCF-7 cells using SRC monoclonal antobody, clone 1F11 (Cat # MAB10395) (green) and negative control (red).
This gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq