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Last updated: 2014/7/28
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SRC monoclonal antibody, clone 1F11

  • Catalog # : MAB10395
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against recombinant SRC.
  • Immunogen:
  • Recombinant protein corresponding to human SRC.
  • Host:
  • Mouse
  • Theoretical MW (kDa):
  • 60
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Recommend Usage:
  • ELISA (1:10000)
    Western Blot (1:500-1:2000)
    Immunohistochemistry (1:200-1:1000)
    Flow cytometry (1:200-1:400)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In ascites (0.03% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis of SRC monoclonal antobody, clone 1F11 (Cat # MAB10395) against MCF-7 (1), A-431 (2), HeLa (3), HEK293 (4), NIH/3T3 (5), PC-12 (6) and COS-7 (7) cell lysate.
  • Western Blot (Recombinant protein)
  • Western Blot (Recombinant protein)
  • Western blot analysis using SRC monoclonal antobody, clone 1F11 (Cat # MAB10395) against human SRC (aa 1-189) recombinant protein. (Expected MW is 47.8 kDa).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical analysis of paraffin-embedded human bladder cancer (A) and human esophageal (B) tissue using SRC monoclonal antobody, clone 1F11 (Cat # MAB10395) with DAB staining.
  • ELISA
  • Flow Cytometry
  • Flow Cytometry
  • Flow cytometric analysis of MCF-7 cells using SRC monoclonal antobody, clone 1F11 (Cat # MAB10395) (green) and negative control (red).
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Western Blot (Recombinant protein)
  • Western Blot (Recombinant protein)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 6714
  • Gene Name:
  • SRC
  • Gene Alias:
  • ASV,SRC1,c-SRC,p60-Src
  • Gene Description:
  • v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian)
  • Gene Summary:
  • This gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000174476,OTTHUMP00000174477,proto-oncogene tyrosine-protein kinase SRC,protooncogene SRC, Rous sarcoma,tyrosine kinase pp60c-src,tyrosine-protein kinase SRC-1
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