SMAD2 monoclonal antibody, clone 5G7
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More Files
- More Functions
Western Blot (Transfected lysate)
Western blot analysis using SMAD2 monoclonal antobody, clone 5G7 (Cat # MAB10391) against HEK293 (1) and SMAD2-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot (Recombinant protein)
Western blot analysis using SMAD2 monoclonal antobody, clone 5G7 (Cat # MAB10391) against recombinant SMAD2 protein.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human cerebellum (A) and human liver cancer (B) tissue using SMAD2 monoclonal antobody, clone 5G7 (Cat # MAB10391) with DAB staining.
Immunofluorescence
Immunofluorescence analysis of U251 cells using SMAD2 monoclonal antobody, clone 5G7 (Cat # MAB10391) (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Flow Cytometry
Flow cytometric analysis of NIH/3T3 cells using SMAD2 monoclonal antobody, clone 5G7 (Cat # MAB10391) (blue) and negative control (red).
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant SMAD2.
Immunogen
Recombinant protein corresponding to human SMAD2.
Host
Mouse
Theoretical MW (kDa)
52
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry (1:200-1:1000)
Immunofluorescence (1:200-1:1000)
Flow cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In ascites (0.03% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis using SMAD2 monoclonal antobody, clone 5G7 (Cat # MAB10391) against HEK293 (1) and SMAD2-hIgGFc transfected HEK293 (2) cell lysate.Western Blot (Recombinant protein)
Western blot analysis using SMAD2 monoclonal antobody, clone 5G7 (Cat # MAB10391) against recombinant SMAD2 protein.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human cerebellum (A) and human liver cancer (B) tissue using SMAD2 monoclonal antobody, clone 5G7 (Cat # MAB10391) with DAB staining.Immunofluorescence
Immunofluorescence analysis of U251 cells using SMAD2 monoclonal antobody, clone 5G7 (Cat # MAB10391) (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Flow cytometric analysis of NIH/3T3 cells using SMAD2 monoclonal antobody, clone 5G7 (Cat # MAB10391) (blue) and negative control (red). -
Gene Info — SMAD2
Entrez GeneID
4087Gene Name
SMAD2
Gene Alias
JV18, JV18-1, MADH2, MADR2, MGC22139, MGC34440, hMAD-2, hSMAD2
Gene Description
SMAD family member 2
Omim ID
601366Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signal of the transforming growth factor (TGF)-beta, and thus regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. This protein is recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation (SARA) protein. In response to TGF-beta signal, this protein is phosphorylated by the TGF-beta receptors. The phosphorylation induces the dissociation of this protein with SARA and the association with the family member SMAD4. The association with SMAD4 is important for the translocation of this protein into the nucleus, where it binds to target promoters and forms a transcription repressor complex with other cofactors. This protein can also be phosphorylated by activin type 1 receptor kinase, and mediates the signal from the activin. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq
Other Designations
MAD, mothers against decapentaplegic homolog 2|Mad protein homolog|Mad, mothers against decapentaplegic homolog 2|Mad-related protein 2|SMAD, mothers against DPP homolog 2|Sma- and Mad-related protein 2|mother against DPP homolog 2
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Interactome
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Pathway
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Disease
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