HDAC3 monoclonal antibody, clone 3A7B5
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More Files
- More Functions
Western Blot
Western blot analysis using HDAC3 monoclonal antibody, clone 3A7B5 (Cat # MAB10374) against truncated Trx-HDAC3 recombinant protein (1), full length HDAC3-hIgGFc (aa1-428) transfected CHO-K1 cell lysate (2) and HeLa cell lysate (3).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human esophagus cancer (A) and breast carcinoma tissue (B), showing nuclear localization. using HDAC3 monoclonal antibody, clone 3A7B5 (Cat # MAB10374) with DAB staining.
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant HDAC3.
Immunogen
Recombinant protein corresponding to amino acids 224-428 of human HDAC3.
Host
Mouse
Theoretical MW (kDa)
48.8
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry (1:200-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In ascites (0.03% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis using HDAC3 monoclonal antibody, clone 3A7B5 (Cat # MAB10374) against truncated Trx-HDAC3 recombinant protein (1), full length HDAC3-hIgGFc (aa1-428) transfected CHO-K1 cell lysate (2) and HeLa cell lysate (3).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human esophagus cancer (A) and breast carcinoma tissue (B), showing nuclear localization. using HDAC3 monoclonal antibody, clone 3A7B5 (Cat # MAB10374) with DAB staining.Enzyme-linked Immunoabsorbent Assay
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Gene Info — HDAC3
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Interactome
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Disease
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