YAP1 monoclonal antibody, clone 1A12
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis using YAP1 monoclonal antobody, clone 1A12 (Cat # MAB10348) against HeLa (1), C6 (2) and COS-7 (3) cell lysate.
Western Blot (Recombinant protein)
Western blot analysis using YAP1 monoclonal antobody, clone 1A12 (Cat # MAB10348) against recombinant human YAP1 protein.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue (A) and rectum cancer tissue (B) using YAP1 monoclonal antobody, clone 1A12 (Cat # MAB10348) with DAB staining.
Flow Cytometry
Flow cytometric analysis of HeLa cells using YAP1 monoclonal antobody, clone 1A12 (Cat # MAB10348) (green) and negative control (red).
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant YAP1.
Immunogen
Recombinant protein corresponding to human YAP1.
Host
Mouse
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry (1:200-1:1000)
Flow cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis using YAP1 monoclonal antobody, clone 1A12 (Cat # MAB10348) against HeLa (1), C6 (2) and COS-7 (3) cell lysate.Western Blot (Recombinant protein)
Western blot analysis using YAP1 monoclonal antobody, clone 1A12 (Cat # MAB10348) against recombinant human YAP1 protein.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue (A) and rectum cancer tissue (B) using YAP1 monoclonal antobody, clone 1A12 (Cat # MAB10348) with DAB staining.Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Flow cytometric analysis of HeLa cells using YAP1 monoclonal antobody, clone 1A12 (Cat # MAB10348) (green) and negative control (red). -
Gene Info — YAP1
Entrez GeneID
10413Gene Name
YAP1
Gene Alias
YAP, YAP2, YAP65, YKI
Gene Description
Yes-associated protein 1, 65kDa
Omim ID
606608Gene Ontology
HyperlinkGene Summary
This gene encodes the human ortholog of chicken YAP protein which binds to the SH3 domain of the Yes proto-oncogene product. This protein contains a WW domain that is found in various structural, regulatory and signaling molecules in yeast, nematode, and mammals, and may be involved in protein-protein interaction. [provided by RefSeq
Other Designations
yes-associated protein 2
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Interactome
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Disease
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