RAF1 monoclonal antibody, clone 4G4
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis using RAF1 monoclonal antobody, clone 4G4 (Cat # MAB10310) against HeLa (1), A-431 (2), COS-7 (3) and C6 (4) cell lysate.
Western Blot (Recombinant protein)
Western blot analysis using RAF1 monoclonal antobody, clone 4G4 (Cat # MAB10310) against recombinant human RAF1 protein.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue using RAF1 monoclonal antobody, clone 4G4 (Cat # MAB10310) with DAB staining.
Flow Cytometry
Flow cytometric analysis of HeLa cells using RAF1 monoclonal antobody, clone 4G4 (Cat # MAB10310) (green) and negative control (red).
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant RAF1.
Immunogen
Recombinant protein corresponding to human RAF1.
Host
Mouse
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry (1:200-1:1000)
Flow cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis using RAF1 monoclonal antobody, clone 4G4 (Cat # MAB10310) against HeLa (1), A-431 (2), COS-7 (3) and C6 (4) cell lysate.Western Blot (Recombinant protein)
Western blot analysis using RAF1 monoclonal antobody, clone 4G4 (Cat # MAB10310) against recombinant human RAF1 protein.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue using RAF1 monoclonal antobody, clone 4G4 (Cat # MAB10310) with DAB staining.Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Flow cytometric analysis of HeLa cells using RAF1 monoclonal antobody, clone 4G4 (Cat # MAB10310) (green) and negative control (red). -
Gene Info — RAF1
Entrez GeneID
5894Gene Name
RAF1
Gene Alias
CRAF, NS5, Raf-1, c-Raf
Gene Description
v-raf-1 murine leukemia viral oncogene homolog 1
Gene Ontology
HyperlinkGene Summary
This gene is the cellular homolog of viral raf gene (v-raf). The encoded protein is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated, the cellular RAF1 protein can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the serine/threonine specific protein kinases, ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2. [provided by RefSeq
Other Designations
Oncogene RAF1|raf proto-oncogene serine/threonine protein kinase
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Interactome
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