PRKDC monoclonal antibody, clone 3H6
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More Files
- More Functions
Western Blot (Transfected lysate)
Western blot analysis using PRKDC monoclonal antobody, clone 3H6 (Cat # MAB10303) against HEK293 (1) and PRKDC-hIgGFc transfected HEK293 (2) cell lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human breast cancer (A), colon cancer (B), lung cancer (C) and brain tissue (D) using PRKDC monoclonal antobody, clone 3H6 (Cat # MAB10303) with DAB staining.
Immunofluorescence
Immunofluorescence analysis of HeLa cells using PRKDC monoclonal antobody, clone 3H6 (Cat # MAB10303) (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant PRKDC.
Immunogen
Recombinant protein corresponding to human PRKDC.
Host
Mouse
Theoretical MW (kDa)
46.9
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry (1:200-1:1000)
Immunofluorescence (1:200-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In ascites (0.03% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis using PRKDC monoclonal antobody, clone 3H6 (Cat # MAB10303) against HEK293 (1) and PRKDC-hIgGFc transfected HEK293 (2) cell lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human breast cancer (A), colon cancer (B), lung cancer (C) and brain tissue (D) using PRKDC monoclonal antobody, clone 3H6 (Cat # MAB10303) with DAB staining.Immunofluorescence
Immunofluorescence analysis of HeLa cells using PRKDC monoclonal antobody, clone 3H6 (Cat # MAB10303) (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.Enzyme-linked Immunoabsorbent Assay
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Gene Info — PRKDC
Entrez GeneID
5591Gene Name
PRKDC
Gene Alias
DNA-PKcs, DNAPK, DNPK1, HYRC, HYRC1, XRCC7, p350
Gene Description
protein kinase, DNA-activated, catalytic polypeptide
Omim ID
600899Gene Ontology
HyperlinkGene Summary
The PRKDC gene encodes the catalytic subunit of a nuclear DNA-dependent serine/threonine protein kinase (DNA-PK). The second component is the autoimmune antigen Ku (MIM 152690), which is encoded by the G22P1 gene on chromosome 22q. On its own, the catalytic subunit of DNA-PK is inactive and relies on the G22P1 component to direct it to the DNA and trigger its kinase activity; PRKDC must be bound to DNA to express its catalytic properties.[supplied by OMIM
Other Designations
hyper-radiosensitivity of murine scid mutation, complementing 1
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Interactome
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Pathway
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Disease
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