Immunohistochemical analysis of paraffin-embedded human breast cancer (A), colon cancer (B), lung cancer (C) and brain tissue (D) using PRKDC monoclonal antobody, clone 3H6 (Cat # MAB10303) with DAB staining.
Immunofluorescence analysis of HeLa cells using PRKDC monoclonal antobody, clone 3H6 (Cat # MAB10303) (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
The PRKDC gene encodes the catalytic subunit of a nuclear DNA-dependent serine/threonine protein kinase (DNA-PK). The second component is the autoimmune antigen Ku (MIM 152690), which is encoded by the G22P1 gene on chromosome 22q. On its own, the catalytic subunit of DNA-PK is inactive and relies on the G22P1 component to direct it to the DNA and trigger its kinase activity; PRKDC must be bound to DNA to express its catalytic properties.[supplied by OMIM
hyper-radiosensitivity of murine scid mutation, complementing 1