Mouse monoclonal antibody raised against partial recombinant EGFR.
Recombinant protein corresponding to amino acids 424-605 of human EGFR.
Protein G purification
Western Blot (1:500-1:1000) The optimal working dilution should be determined by the end user.
In PBS, pH 7.4 (0.1% sodium azide)
Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Western Blot (Cell lysate)
Western blot analysis of HaCaT cell extracts (35 ug) with EGFR monoclonal antibody, clone AT6E3 (Cat # MAB10013) at 1:500 (Lane 1) and 1:1000 (Lane 2) dilution. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. [provided by RefSeq
avian erythroblastic leukemia viral (v-erb-b) oncogene homolog,cell growth inhibiting protein 40,cell proliferation-inducing protein 61,epidermal growth factor receptor