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Last updated: 2014/7/21
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ESR1 monoclonal antibody, clone mAN1

  • Catalog # : MAB0118
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against synthetic peptide of ESR1.
  • Immunogen:
  • A synthetic peptide (conjugated with KLH) corresponding to amino acids 19-32 of human ESR1.
  • Host:
  • Mouse
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Isotype:
  • IgG3, kappa
  • Recommend Usage:
  • The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (0.01% thimerosal)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains thimerosal: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • ChIP
  • ChIP
  • ChIP results obtained with the ESR1 monoclonal antibody, clone mAN1 (Cat # MAB0118). ChIP assays were performed using MCF-7 cells.
  • Western Blot (Recombinant protein)
  • Western Blot (Recombinant protein)
  • Analysis by Western blot of ESR1 using the ESR1 monoclonal antibody, clone mAN1 (Cat # MAB0118). Specificity of the antibody is assessed by Western blot using recombinant ESR1 (right side) and recombinant ESR2 (left side), 100 fmol each per well. The monoclonal antibody directed against ESR1 monoclonal antibody, clone mAN1 (Cat # MAB0118) is used at 7 ug/mL . The antibody is clearly recognizing the ESR1 isoform and not ESR2. (Note that the antibody is not recommended for use in WB for detection of endogenous low levels of ESR expression.)
  • Immunocytochemistry
  • Immunocytochemistry
  • Immunocytochemistry using the ESR1 monoclonal antibody, clone mAN1 (Cat # MAB0118). COS-7 cells transiently overexpressing human ESR1 (left panel) or ESR2 (right panel) were both labeled with the ESR1 monoclonal antibody, clone mAN1 (Cat # MAB0118) followed by biotinylated second antibody and peroxidase-labeled avidin. The monoclonal antibody directed against ESR1 is used at 15 ug/mL . The antibody is clearly recognizing the ESR1 isoform and not ESR2.
  • Immunoprecipitation
  • ELISA
  • Application Image
  • Western Blot (Recombinant protein)
  • Western Blot (Recombinant protein)
  • enlarge
  • Immunoprecipitation
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 2099
  • Gene Name:
  • ESR1
  • Gene Alias:
  • DKFZp686N23123,ER,ESR,ESRA,Era,NR3A1
  • Gene Description:
  • estrogen receptor 1
  • Gene Summary:
  • This gene encodes an estrogen receptor, a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. The protein localizes to the nucleus where it may form a homodimer or a heterodimer with estrogen receptor 2. Estrogen and its receptors are essential for sexual development and reproductive function, but also play a role in other tissues such as bone. Estrogen receptors are also involved in pathological processes including breast cancer, endometrial cancer, and osteoporosis. Alternative splicing results in several transcript variants, which differ in their 5' UTRs and use different promoters. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000017718,OTTHUMP00000017719,estrogen receptor alpha
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