Western blot analysis of TLR3 in lysates from untransfected 293 cells (lane A), 293 cells transfected with human TLR3 cDNA (lane B), and 20 ug/lane human intestine tissue lysate (lane C). Using TLR3 monoclonal antibody, clone 40C1285.6 (Cat # MAB0088).
IHC analysis of TLR3 in (A) human gut lumen (longitudinal section, transverse region). Using TLR3 monoclonal antibody, clone 40C1285.6 (Cat # MAB0088) at 10 ug/mL (Data courtesy Dr. Elizabeth Furrie, University of Dundee) and mouse spleen tissue using isotype control (B) and TLR3 monoclonal antibody, clone 40C1285.6 (Cat # MAB0088) (C) at 5 mg/mL .
Intracellular flow analysis of TLR3 in Ramos cells. Using 2 ug of TLR3 monoclonal antibody, clone 40C1285.6 (Cat # MAB0088). Shaded histogram represents Ramos cells without antibody; Green represents isotype control; red represents anti-TLR3 antibody.
The protein encoded by this gene is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This receptor is most abundantly expressed in placenta and pancreas, and is restricted to the dendritic subpopulation of the leukocytes. It recognizes dsRNA associated with viral infection, and induces the activation of NF-kappaB and the production of type I interferons. It may thus play a role in host defense against viruses. Use of alternative polyadenylation sites to generate different length transcripts has been noted for this gene. [provided by RefSeq