IKBKB monoclonal antibody, clone 10AG2
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of 30 ug of total cell lysate from A) Daudi, B) HeLa, and C) mouse NIH/3T3 cells. Using IKBKB monoclonal antibody, clone 10AG2 (Cat # MAB0075) at 2 ug/mL dilution.
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Specification
Product Description
Mouse monoclonal antibody raised against full length recombinant IKBKB.
Immunogen
Recombinant protein corresponding to full length human IKBKB.
Host
Mouse
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
The optimal working dilution should be determined by the end user.
Storage Buffer
In PBS (0.05% BSA, 0.05% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of 30 ug of total cell lysate from A) Daudi, B) HeLa, and C) mouse NIH/3T3 cells. Using IKBKB monoclonal antibody, clone 10AG2 (Cat # MAB0075) at 2 ug/mL dilution.Immunoprecipitation
Flow Cytometry
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Gene Info — IKBKB
Entrez GeneID
3551Gene Name
IKBKB
Gene Alias
FLJ40509, IKK-beta, IKK2, IKKB, MGC131801, NFKBIKB
Gene Description
inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase beta
Omim ID
603258Gene Ontology
HyperlinkGene Summary
NFKB1 (MIM 164011) or NFKB2 (MIM 164012) is bound to REL (MIM 164910), RELA (MIM 164014), or RELB (MIM 604758) to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA, MIM 164008, or NFKBIB, MIM 604495), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, MIM 600664, or IKBKB) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T; R is an A or G purine; and Y is a C or T pyrimidine).[supplied by OMIM
Other Designations
inhibitor of nuclear factor kappa B kinase beta subunit|nuclear factor NF-kappa-B inhibitor kinase beta
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Interactome
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Pathway
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Disease
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Publication Reference
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TRAF6 ubiquitin ligase is essential for RANKL signaling and osteoclast differentiation.
Lamothe B, Webster WK, Gopinathan A, Besse A, Campos AD, Darnay BG.
Biochemical and Biophysical Research Communications 2007 Jun; 359(4):1044.
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The anti-inflammatory effects of selenium are mediated through 15-deoxy-Delta12,14-prostaglandin J2 in macrophages.
Vunta H, Davis F, Palempalli UD, Bhat D, Arner RJ, Thompson JT, Peterson DG, Reddy CC, Prabhu KS.
The Journal of Biological Chemistry 2007 Apr; 282(25):17964.
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Hepatocyte-specific IKK gamma/NEMO expression determines the degree of liver injury.
Beraza N, Ludde T, Assmus U, Roskams T, Vander Borght S, Trautwein C.
Gastroenterology 2007 Apr; 132(7):2504.
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Evidence that TNF-TNFR1-TRADD-TRAF2-RIP-TAK1-IKK pathway mediates constitutive NF-kappaB activation and proliferation in human head and neck squamous cell carcinoma.
Jackson-Bernitsas DG, Ichikawa H, Takada Y, Myers JN, Lin XL, Darnay BG, Chaturvedi MM, Aggarwal BB.
Oncogene 2006 Sep; 26(10):1385.
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Indirubin enhances tumor necrosis factor-induced apoptosis through modulation of nuclear factor-kappa B signaling pathway.
Gautam Sethi, Kwang Seok Ahn, Santosh K Sandur, Xin Lin, Madan M Chaturvedi, Bharat B Aggarwal.
The Journal of Biological Chemistry 2006 Aug; 281(33):23425.
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Skin lesion development in a mouse model of incontinentia pigmenti is triggered by NEMO deficiency in epidermal keratinocytes and requires TNF signaling.
Arianna Nenci, Marion Huth, Alfred Funteh, Marc Schmidt-Supprian, Wilhelm Bloch, Daniel Metzger, Pierre Chambon, Klaus Rajewsky, Thomas Krieg, Ingo Haase, Manolis Pasparakis.
Human Molecular Genetics 2006 Feb; 15(4):531.
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Suberoylanilide hydroxamic acid potentiates apoptosis, inhibits invasion, and abolishes osteoclastogenesis by suppressing nuclear factor-kappaB activation.
Yasunari Takada, Ann Gillenwater, Haruyo Ichikawa, Bharat B Aggarwal.
The Journal of Biological Chemistry 2006 Mar; 281(9):5612.
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Diminished expression of transcription factors nuclear factor kappaB and CCAAT/enhancer binding protein underlies a novel tumor evasion mechanism affecting macrophages of mammary tumor-bearing mice.
Marta Torroella-Kouri, Xiaojing Ma, Giselle Perry, Milena Ivanova, Pedro J Cejas, Jennifer L Owen, Vijaya Iragavarapu-Charyulu, Diana M Lopez.
Cancer Research 2005 Nov; 65(22):10578.
Application：WB-Ce, Mouse, Mouse peritoneal macrophages.
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IKK mediates ischemia-induced neuronal death.
Oliver Herrmann, Bernd Baumann, Rossana de Lorenzi, Sajjad Muhammad, Wen Zhang, Jens Kleesiek, Max Malfertheiner, Martin Köhrmann, Ioana Potrovita, Ira Maegele, Cordian Beyer, James R Burke, Mazahir T Hasan, Hermann Bujard, Thomas Wirth, Manolis Pasparakis, Markus Schwaninger.
Nature Medicine 2005 Dec; 11(12):1322.
Application：IHC-P, WB-Ce, WB-Ti, Mouse, Mouse brain, Mouse cortical neurons, Mouse forebrain.
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N-(4-hydroxyphenyl)retinamide inhibits invasion, suppresses osteoclastogenesis, and potentiates apoptosis through down-regulation of I(kappa)B(alpha) kinase and nuclear factor-kappaB-regulated gene products.
Shishir Shishodia, Angelica M Gutierrez, Reuben Lotan, Bharat B Aggarwal.
Cancer Research 2005 Oct; 65(20):9555.
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Curcumin (diferuloylmethane) inhibits constitutive NF-kappaB activation, induces G1/S arrest, suppresses proliferation, and induces apoptosis in mantle cell lymphoma.
Shishir Shishodia, Hesham M Amin, Raymond Lai, Bharat B Aggarwal.
Biochemical Pharmacology 2005 Sep; 70(5):700.
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Identification of a novel blocker of I kappa B alpha kinase that enhances cellular apoptosis and inhibits cellular invasion through suppression of NF-kappa B-regulated gene products.
Haruyo Ichikawa, Yasunari Takada, Akira Murakami, Bharat B Aggarwal.
The Journal of Immunology 2005 Jun; 174(11):7383.
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Deletion of IKK2 in hepatocytes does not sensitize these cells to TNF-induced apoptosis but protects from ischemia/reperfusion injury.
Tom Luedde, Ulrike Assmus, Torsten Wüstefeld, Andreas Meyer zu Vilsendorf, Tania Roskams, Mark Schmidt-Supprian, Klaus Rajewsky, David A Brenner, Michael P Manns, Manolis Pasparakis, Christian Trautwein.
The Journal of Clinical Investigation 2005 Apr; 115(4):849.
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Ectoderm-targeted overexpression of the glucocorticoid receptor induces hypohidrotic ectodermal dysplasia.
Jose Luis Cascallana, Ana Bravo, Eva Donet, Hugo Leis, Maria Fernanda Lara, Jesus M Paramio, Jose L Jorcano, Paloma Perez.
Endocrinology 2005 Jun; 146(6):2629.
Application：IHC-P, Mouse, Mouse embryo.
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Guggulsterone inhibits NF-kappaB and IkappaBalpha kinase activation, suppresses expression of anti-apoptotic gene products, and enhances apoptosis.
Shishodia S, Aggarwal BB.
The Journal of Biological Chemistry 2004 Nov; 279(45):47148.
Application：WB, Human, H1299 cells.
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Cyclooxygenase (COX)-2 inhibitor celecoxib abrogates TNF-induced NF-kappa B activation through inhibition of activation of I kappa B alpha kinase and Akt in human non-small cell lung carcinoma: correlation with suppression of COX-2 synthesis.
Shishodia S, Koul D, Aggarwal BB.
Journal of Immunology 2004 Aug; 173(3):2011.
Application：WB, Human, H1299 cells.
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Genetic deletion of glycogen synthase kinase-3beta abrogates activation of IkappaBalpha kinase, JNK, Akt, and p44/p42 MAPK but potentiates apoptosis induced by tumor necrosis factor.
Takada Y, Fang X, Jamaluddin MS, Boyd DD, Aggarwal BB.
The Journal of Biological Chemistry 2004 Sep; 279(38):39541.
Application：WB, Mouse, MEFs.
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Protein farnesyltransferase inhibitor (SCH 66336) abolishes NF-kappaB activation induced by various carcinogens and inflammatory stimuli leading to suppression of NF-kappaB-regulated gene expression and up-regulation of apoptosis.
Yasunari Takada, Fadlo R Khuri, Bharat B Aggarwal.
The Journal of Biological Chemistry 2004 Jun; 279(25):26287.
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Identification of a p65 peptide that selectively inhibits NF-kappa B activation induced by various inflammatory stimuli and its role in down-regulation of NF-kappaB-mediated gene expression and up-regulation of apoptosis.
Takada Y, Singh S, Aggarwal BB.
The Journal of Biological Chemistry 2004 Apr; 279(15):1096.
Application：IP, WB, Human, HEK 293 cells.
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Flavopiridol inhibits NF-kappaB activation induced by various carcinogens and inflammatory agents through inhibition of IkappaBalpha kinase and p65 phosphorylation: abrogation of cyclin D1, cyclooxygenase-2, and matrix metalloprotease-9.
Yasunari Takada, Bharat B Aggarwal.
The Journal of Biological Chemistry 2004 Feb; 279(6):4750.
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Glucocorticoid Receptor Counteracts Tumorigenic Activity of Akt in Skin through Interference with the Phosphatidylinositol 3-Kinase Signaling Pathway.
Hugo Leis, Angustias Page, Angel Ramirez, Ana Bravo, Carmen Segrelles, Jesus Paramio, Domingo Barettino, Jose L Jorcano, Paloma Perez.
Molecular Endocrinology (Baltimore, Md.) 2004 Feb; 18(2):303.
Application：WB-Ti, Mouse, Mouse dorsal skin.
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IKKepsilon and TBK1 are essential components of the IRF3 signaling pathway.
Fitzgerald KA, McWhirter SM, Faia KL, Rowe DC, Latz E, Golenbock DT, Coyle AJ, Liao SM, Maniatis T.
Nature Immunology 2003 May; 4(5):491.
Application：IP, WB-Tr, Human, HEK 293T cells.
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Adenosine suppresses activation of nuclear factor-kappaB selectively induced by tumor necrosis factor in different cell types.
Majumdar S, Aggarwal BB.
Oncogene 2003 Feb; 22(8):1206.
Application：WB-Ce, Human, KBM-5 cells.
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Impaired NF-kappa B activation and increased production of tumor necrosis factor alpha in transgenic mice expressing keratin K10 in the basal layer of the epidermis.
Mirentxu Santos, Paloma Perez, Carmen Segrelles, Sergio Ruiz, Jose L Jorcano, Jesus M Paramio.
The Journal of Biological Chemistry 2003 Apr; 278(15):13422.
Application：WB-Ti, WB-Tr, Mouse, Mouse epidermis, Mouse keratinocytes.
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Piceatannol inhibits TNF-induced NF-kappaB activation and NF-kappaB-mediated gene expression through suppression of IkappaBalpha kinase and p65 phosphorylation.
Kazuhiro Ashikawa, Sekhar Majumdar, Sanjeev Banerjee, Alok C. Bharti, Shishir Shishodia, Bharat B. Aggarwal
The Journal of Immunology 2002 Dec; 169(11):6490.
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A recessive mutant cell line with a constitutive IkappaB kinase activity.
Chinanonwait N, Miura H, Yamamoto N, Yamaoka S.
FEBS Letters 2002 Nov; 531(3):553.
Application：WB-Tr, Rat, Rat-1 fibroblasts.
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Cigarette smoke condensate activates nuclear transcription factor-kappaB through phosphorylation and degradation of IkappaB(alpha): correlation with induction of cyclooxygenase-2.
Anto RJ, Mukhopadhyay A, Shishodia S, Gairola CG, Aggarwal BB.
Carcinogenesis 2002 Sep; 23(9):1511.
Application：KA, WB-Ce, Human, U-937 cells.
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Thalidomide suppresses NF-kappa B activation induced by TNF and H2O2, but not that activated by ceramide, lipopolysaccharides, or phorbol ester.
Sekhar Majumdar, Betty Lamothe, Bharat B Aggarwal.
The Journal of Immunology 2002 Mar; 168(6):2644.
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Methotrexate suppresses NF-kappaB activation through inhibition of IkappaBalpha phosphorylation and degradation.
S Majumdar, B B Aggarwal.
The Journal of Immunology 2001 Sep; 167(5):2911.
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Complete reconstitution of human IkappaB kinase (IKK) complex in yeast. Assessment of its stoichiometry and the role of IKKgamma on the complex activity in the absence of stimulation.
Miller BS, Zandi E.
The Journal of Biological Chemistry 2001 Sep; 276(39):36320.
Application：WB-Ce, WB-Tr, Human, Yeast, HeLa cells, Saccharomyces cerevisiae.
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Leflunomide suppresses TNF-induced cellular responses: effects on NF-kappa B, activator protein-1, c-Jun N-terminal protein kinase, and apoptosis.
S K Manna, A Mukhopadhyay, B B Aggarwal.
The Journal of Immunology 2000 Nov; 165(10):5962.
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TRAF6 ubiquitin ligase is essential for RANKL signaling and osteoclast differentiation.
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