TLR4 monoclonal antibody, clone HTA125
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More Files
- More Functions
Flow Cytometry
Flow cytometric analysis of TLR4 using TLR4 monoclonal antibody, clone HTA125 (Cat # MAB0071) on PBMCs at 2ug/ 1X10^6 cells. M1 gate are unstained PBMC.
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Specification
Product Description
Mouse monoclonal antibody raised against full length recombinant TLR4.
Immunogen
Recombinant protein corresponding to full length human TLR4.
Host
Mouse
Reactivity
Human
Form
Liquid
Isotype
IgG2a
Recommend Usage
The optimal working dilution should be determined by the end user.
Storage Buffer
In PBS (0.05% BSA, 0.05% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunocytochemistry
Immunofluorescence
Immunoprecipitation
Flow Cytometry
Flow cytometric analysis of TLR4 using TLR4 monoclonal antibody, clone HTA125 (Cat # MAB0071) on PBMCs at 2ug/ 1X10^6 cells. M1 gate are unstained PBMC. -
Gene Info — TLR4
Entrez GeneID
7099Gene Name
TLR4
Gene Alias
ARMD10, CD284, TOLL, hToll
Gene Description
toll-like receptor 4
Omim ID
603030Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This receptor is most abundantly expressed in placenta, and in myelomonocytic subpopulation of the leukocytes. It has been implicated in signal transduction events induced by lipopolysaccharide (LPS) found in most gram-negative bacteria. Mutations in this gene have been associated with differences in LPS responsiveness. Also, several transcript variants of this gene have been found, but the protein coding potential of most of them is uncertain. [provided by RefSeq
Other Designations
OTTHUMP00000022807|homolog of Drosophila toll
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Interactome
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Pathway
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Disease
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Publication Reference
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TLR2 and TLR4 expression on the immune cells of tuberculous pleural fluid.
Prabha C, Rajashree P, Sulochana DD.
Immunology Letters 2007 Dec; 117(1):26.
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A cellular micro-RNA, let-7i, regulates Toll-like receptor 4 expression and contributes to cholangiocyte immune responses against Cryptosporidium parvum infection.
Chen XM, Splinter PL, O'Hara SP, LaRusso NF.
The Journal of Biological Chemistry 2007 Jul; 282(39):28929.
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Intracellular signaling mechanisms regulating toll-like receptor-mediated activation of eosinophils.
Wong CK, Cheung PF, Ip WK, Lam CW.
American Journal of Respiratory Cell and Molecular Biology 2007 Mar; 37(1):85.
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Execution of macrophage apoptosis by PE_PGRS33 of Mycobacterium tuberculosis is mediated by Toll-like receptor 2-dependent release of tumor necrosis factor-alpha.
Basu S, Pathak SK, Banerjee A, Pathak S, Bhattacharyya A, Yang Z, Talarico S, Kundu M, Basu J.
The Journal of Biological Chemistry 2006 Nov; 282(2):1039.
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RANTES modulates TLR4-induced cytokine secretion in human peripheral blood monocytes.
Shahrara S, Park CC, Temkin V, Jarvis JW, Volin MV, Pope RM.
Journal of Immunology 2006 Oct; 177(8):5077.
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In celiac disease, a subset of autoantibodies against transglutaminase binds toll-like receptor 4 and induces activation of monocytes.
Giovanna Zanoni, Riccardo Navone, Claudio Lunardi, Giuseppe Tridente, Caterina Bason, Simona Sivori, Ruggero Beri, Marzia Dolcino, Enrico Valletta, Roberto Corrocher, Antonio Puccetti.
PLoS Medicine 2006 Sep; 3(9):e358.
Application：ELISA, Human, Supernatant of cultured monocytes.
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Regulation of the polymeric Ig receptor by signaling through TLRs 3 and 4: linking innate and adaptive immune responses.
Tracey A Schneeman, Maria E C Bruno, Hilde Schjerven, Finn-Eirik Johansen, Laura Chady, Charlotte S Kaetzel.
The Journal of Immunology 2005 Jul; 175(1):376.
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Expression of mRNA and proteins for toll-like receptors, associated molecules, defensins and LL-37 by SRIK-NKL, a CD8+ NK/T cell line.
Srivastava MD, Srivastava BI.
Leukemia Research 2005 Jul; 29(7):813.
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Toll-like receptor expression in human keratinocytes: nuclear factor kappaB controlled gene activation by Staphylococcus aureus is toll-like receptor 2 but not toll-like receptor 4 or platelet activating factor receptor dependent.
Mempel M, Voelcker V, Kollisch G, Plank C, Rad R, Gerhard M, Schnopp C, Fraunberger P, Walli AK, Ring J, Abeck D, Ollert M.
The Journal of Investigative Dermatology 2003 Dec; 121(6):1389.
Application：IF, Human, Human keratinocytes.
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Highly purified lipoteichoic acid activates neutrophil granulocytes and delays their spontaneous apoptosis via CD14 and TLR2.
Lotz S, Aga E, Wilde I, van Zandbergen G, Hartung T, Solbach W, Laskay T.
Journal of Leukocyte Biology 2004 Mar; 75(3):467.
Application：Func, IA, Human, Neutrophils.
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TLR2 and TLR4 expression on the immune cells of tuberculous pleural fluid.
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